Bacillus velezensis Spore Composition for Heat-Stable Probiotics
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Solution Overview
Problem
Existing probiotics, such as Lactobacilluseae and Bifidobacterium, are not stable under high temperatures and acidic or basic conditions, limiting their use in various food and beverage products, and there is a need for effective probiotics that can promote gut microbiota balance, intestinal epithelial barrier integrity, and gut immune health.
Innovation Solution
The use of Bacillus velezensis strain BV379, which forms spores that are stable under high temperatures and various pH conditions, producing bioactive molecules like macrolactins to inhibit pathogenic bacteria and promote the growth of beneficial microbes, thereby supporting digestive and gastrointestinal health.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If existing probiotics such as Lactobacilluseae and Bifidobacterium are used, then they can promote gut microbiota balance and intestinal health, but they are not stable under high temperatures and acidic or basic conditions
Solution Approach 1:
The patent changes the biological form of the probiotic from vegetative cells to spores, which fundamentally alters the stability parameters. Spores exhibit enhanced resistance to high temperatures (stable at pasteurization and sterilization temperatures) and extreme pH conditions (stable at pH 2-12) compared to vegetative cells, while maintaining probiotic functionality upon germination in the gastrointestinal tract
Solution Approach 2:
The patent creates a composite structure by forming spores that contain both the probiotic microorganism and protective spore wall components. This composite spore structure provides inherent protection against environmental stressors including heat, acid, and oxidative conditions, while still allowing the probiotic to exert its beneficial effects in the gut
2Stability of the object's composition
If Bacillus velezensis strain BV379 spores are used, then stability under high temperatures and acidic conditions is improved, but the complexity of spore formation and metabolite production increases
Solution Approach 1:
The Bacillus velezensis strain BV379 performs self-service by autonomously forming spores and producing protective metabolites (macrolactins, fengycin, iturin) through its own metabolic pathways. This eliminates the need for external protective agents or complex formulation processes, as the bacteria self-organize into stable spore structures with inherent protective capabilities
Solution Approach 2:
The spore-forming capability serves multiple functions simultaneously: it provides thermal stability, acid resistance, oxidative stress protection, and facilitates controlled release in the gastrointestinal tract. The metabolites produced serve dual roles as both protective agents during storage and active probiotic components that inhibit pathogenic bacteria and support gut health
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Bacillus velezensis strain BV379 maintains gut microbiota balance, enhances intestinal barrier integrity, and supports immune health by inhibiting pathogenic bacteria and promoting beneficial microbes, even in high-temperature and acidic environments.
Implementation Method 1
Existing probiotics, such as Lactobacilluseae and Bifidobacterium, are not stable under high temperatures and acidic or basic conditions... Bacillus velezensis strain BV379, which forms spores that are stable under high temperatures and various pH conditions
Implementation Method 2
producing bioactive molecules like macrolactins to inhibit pathogenic bacteria
Data Source
AI summary
A spore-forming Bacillus species, and more particularly, a Bacillus velezensis strain identified as BV379 is provided. Compositions comprising BV379 and methods of using the same are also provided.