Bacillus velezensis XG3 Agent for Angelica sinensis Bolting
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Solution Overview
Problem
The issue of early bolting in Angelica sinensis plants is prevalent, leading to reduced yield and quality, and existing methods fail to effectively prevent it early in the growth process.
Innovation Solution
A Bacillus velezensis XG3 strain is isolated, cultured, and formulated into a microbial agent using a specific method, which is applied to promote seed germination and plant growth, and prevent early bolting in Angelica sinensis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional field management methods are used to control early bolting, then the planting process is simple and easy to operate, but the early bolting rate remains high (30-80%) and yield is seriously affected
Solution Approach 1:
The patent applies preliminary action by introducing the Bacillus velezensis XG3 microbial agent to seedlings at the seedling stage (3-5 true leaves) or earlier (seed soaking, root irrigation). This early intervention establishes protective microbial communities before bolting occurs, enabling prevention rather than treatment. The microbial agent colonizes the rhizosphere and plant surface, creating a protective barrier that suppresses bolting hormones and promotes vegetative growth, thereby preventing the harmful effect before it manifests.
2Measurement precision
If seedling selection is performed to remove big seedlings that are easy to bolt, then some problematic seedlings are removed, but early bolting can only be detected after bolting occurs, making it too late to replenish seedlings
Solution Approach 1:
The microbial agent performs preliminary action by preventing bolting before it occurs, rather than detecting and responding after bolting is visible. By applying the agent at the seedling stage, the system proactively suppresses the bolting process through microbial colonization and biochemical regulation, eliminating the need for late-stage detection and replacement of bolted seedlings.
3Productivity
If planting quantity is increased to compensate for early bolting losses, then yield impact is reduced, but high planting density easily causes more early bolting
Solution Approach 1:
The patent converts the harmful effect of high planting density (which normally promotes early bolting through competition and stress) into a benefit. The Bacillus velezensis XG3 microbial agent modifies the plant-microbe-soil interactions under high density conditions, transforming the competitive stress that would normally induce bolting into conditions that promote vegetative growth. The microbial agent produces substances that suppress bolting hormones and enhance vegetative development, thereby converting the harmful density effect into a beneficial outcome where high density no longer leads to high bolting rates.
4Object-affected harmful factors
If microbial agents are applied instead of traditional fertilizers and pesticides, then pesticide residue accumulation is reduced and green planting is promoted, but the complexity of microbial agent preparation and application increases
Solution Approach 1:
The Bacillus velezensis XG3 microbial agent applies self-service by performing multiple functions simultaneously: it colonizes the plant rhizosphere, suppresses bolting through biochemical regulation, promotes vegetative growth, and protects against pathogens. This multi-functionality eliminates the need for separate applications of fertilizers, pesticides, and growth regulators, thereby reducing overall system complexity despite the advanced nature of the microbial agent itself.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The microbial agent effectively delays flowering, increases yield, and controls early bolting, contributing to sustainable and green cultivation practices.
Implementation Method 1
inoculating the Bacillus velezensis XG3 strain into an LB liquid culture medium, performing activation culture on a thermostatic shaker, then transferring the activated strain to a triangular flask containing an LB liquid culture medium for fermentation
Implementation Method 2
The Bacillus velezensis XG3 strain obtained by separation can be used for promoting seed germination and plant growth of Angelica sinensis
Implementation Method 3
delaying the flowering of Arabidopsis thaliana, and the prepared microbial agent can increase the yield of Angelica sinensis and prevent and control early bolting of Angelica sinensis
Data Source
AI summary
A strain separation method for preventing and controlling early bolting of Angelica sinensis, and preparation of a microbial agent and use thereof, the strain is obtained by separation, purification and cultivation from rhizosphere soil of Angelica sinensis, and is identified as Bacillus spp. by Microbial 16S rDNA sequencing; the strain was deposited at China Center for Type Culture Collection on Jun. 24, 2021 under CCTCC NO: M 2021767; tThe Bacillus velezensis XG3 strain provided by the present invention can promote the seed germination of Angelica sinensis and delay the flowering of Arabidopsis thaliana for about 2 days; according to field test verification, the microbial agent can increase the root weight, root diameter and rootlet number of Angelica sinensis, can effectively prevent and control early bolting of Angelica sinensis.