Bacillus Methylotrophicus XT1 for Root-Zone Growth and Control

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Solution Overview

Problem

There is a pressing need for alternative and more effective strategies to stimulate plant growth and biologically control phytopathogens such as bacteria, fungi, and nematodes, while minimizing environmental impact and avoiding resistance to chemical treatments.

Innovation Solution

The use of Bacillus methylotrophicus strain XT1, deposited as CECT8661, which produces spores, biofilms, and various enzymes and compounds that enhance plant growth and inhibit pathogens, combined with localized irrigation systems for application.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical treatments are used to control phytopathogens and stimulate plant growth, then plant protection and growth stimulation are achieved, but environmental contamination and development of resistance occur

Engineering Contradiction:
Improveplant protection effectivenessVSAvoidenvironmental contamination
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful effect of chemical pesticides into a beneficial biological control system by using beneficial microorganisms (Bacillus subtilis, Pseudomonas fluorescens, Trichoderma harzianum) that produce antibiotics and compete with phytopathogens, thereby protecting plants without chemical contamination

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The patent introduces intermediary biological agents (beneficial bacteria and fungi) that mediate between the plant and phytopathogens, providing protection through biological mechanisms such as antibiotic production, competition for nutrients, and induction of plant resistance rather than direct chemical action

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If chemical pesticides are applied to control phytopathogens, then disease incidence is reduced, but pollinating insects and animal health are negatively affected

Engineering Contradiction:
Improvedisease controlVSAvoidimpact on beneficial organisms
Core Design Contradiction:
ReliabilityVSObject-generated harmful factors

Solution Approach 1:

The patent transforms the harmful impact on beneficial organisms into a beneficial outcome by using selective biological control agents that specifically target phytopathogens while leaving pollinating insects and other beneficial organisms unaffected, thereby protecting both plants and ecosystem health

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

3Ease of operation

If conventional irrigation systems are used for applying bacterial treatments, then application is simple, but treatment effectiveness is reduced due to dilution and non-targeted distribution

Engineering Contradiction:
Improveapplication simplicityVSAvoidtreatment effectiveness
Core Design Contradiction:
Ease of operationVSReliability

Solution Approach 1:

The patent applies bacterial treatments locally at the root zone through subsurface irrigation systems, concentrating the beneficial microorganisms where they are most needed (in the rhizosphere) rather than distributing them widely through conventional overhead irrigation, thereby enhancing treatment effectiveness while maintaining ease of operation

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The patent uses hydraulic systems (subsurface irrigation) to deliver bacterial treatments directly to the root zone through underground pipes and emitters, leveraging fluid pressure and flow control to achieve targeted application without the dilution and waste associated with conventional spray systems

Inventive Principle:
Principle #29Pneumatics and hydraulics

Data Source

PatentEP3178325B1Use of bacillus methylotrophicus strain XT1 as a stimulant of plant growth and biological control means, and isolates of said species
Publication Date: 2025.09.03 UNIV DE GRANADA
  • EP3178325B1 patent drawingFigure 1~2
  • EP3178325B1 patent drawingFigure 3~4
  • EP3178325B1 patent drawingFigure 5~7

AI summary

The invention relates to the use of microorganisms as plant growth stimulants and for the biological control of bacterium, insects, fungi and phytopathogenic nematodes. More specifically, the invention relates to the use of microorganisms of the genus Bacillus, more specifically the Bacillus methylotrophicus species, as well as to cultures thereof, compositions comprising these bacteria, different culture methods and the products comprising same, as plant growth stimulants and for the biological control of bacterium, insects, fungi and phytopathogenic nematodes.