Backscatter Diffractogram Microorganism Identification

The patent inverts the observation configuration from transmission mode to backscatter mode. Instead of placing the detector behind the sample to capture transmitted light, the detector is positioned to capture light scattered back from the sample. This inversion allows observation through opaque and colored media that would block transmitted light, while maintaining relative simplicity of the identification method.

The patent introduces a diffusing screen as an intermediary element between the sample and the detector. This screen converts the scattered light into a visible diffractogram pattern that can be captured by the detector. The screen acts as a mediator that makes the backscattered radiation observable and analyzable, enabling identification of microorganisms in opaque media without requiring complex instrumentation.

The patent creates an optical copy or representation of the microorganism's scattering properties in the form of a diffractogram on a screen. Instead of using complex spectrometric equipment to directly analyze the sample, the system projects a visual pattern that replicates the scattering characteristics, which can then be analyzed by simple imaging detectors. This copying approach maintains identification accuracy while eliminating the need for costly specialized equipment.

The patent replaces expensive, complex spectrometric instruments with simple, inexpensive components: a light source, a diffusing screen, and a basic image detector. These inexpensive components can be easily manufactured and disposed of, providing high-accuracy identification capability without the burden of maintaining costly equipment or requiring specialized operator training.

The patent uses periodic or pulsed illumination with a light source to illuminate the sample and generate backscattered radiation patterns. By using controlled illumination pulses rather than continuous lighting, the system achieves rapid data acquisition while maintaining equipment simplicity. This periodic action enables fast identification without requiring complex continuous monitoring systems.

The patent replaces mechanical or chemical identification processes with optical detection. Instead of using slow colorimetric chemical reactions or manual morphological analysis, the system uses optical backscatter detection to rapidly capture diffractogram patterns. This substitution of optical detection for mechanical/chemical methods maintains equipment simplicity while dramatically increasing identification speed to match clinical productivity requirements.