Bacterial Biocalcification for Non-Toxic Construction Materials

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Solution Overview

Problem

Conventional construction and industrial materials often contain toxic chemicals, and there is a lack of sustainable, non-toxic alternatives that can be effectively integrated into architectural and industrial product design, particularly in the use of bacterial biocalcification methods for structural applications.

Innovation Solution

A method utilizing bacterial biocalcification to solidify sand and fibers into a biodegradable composite structure through calcium carbonate sedimentation, where bacteria such as Sporosarcina pasteurii are immobilized onto a biodegradable polymer composite, enabling the production of non-toxic, ecological bio-design products using 3D printing technology.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Object-affected harmful factors

If conventional production methods using ceramics, wood, concrete, cement and petrochemicals are used, then structural strength and durability are achieved, but toxic materials are introduced into the environment and human health is compromised

Engineering Contradiction:
ImprovetoxicityVSAvoidstructural strength
Core Design Contradiction:
Object-affected harmful factorsVSStrength

Solution Approach 1:

The patent changes the chemical parameters of construction materials by replacing traditional cement-based binders with bacterial biocalcification systems. The bacteria (e.g., Sporosarcina pasteurii) convert urea and calcium lactate into calcium carbonate through metabolic processes, creating a binding mechanism that eliminates toxic chemicals while maintaining structural integrity. This parameter change transforms the material composition from harmful to benign.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent replaces the chemical binding mechanism of conventional cement with a biological mechanism. Instead of using chemical reactions in cement hydration, the system employs bacterial metabolism and enzymatic activity (urease production) to precipitate calcium carbonate and bind aggregates. This substitution of biological processes for chemical processes eliminates toxic emissions while achieving comparable structural strength.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Object-affected harmful factors

If bacterial biocalcification method is used to produce non-toxic materials, then environmental friendliness and non-toxicity are achieved, but manufacturing complexity and process integration challenges increase

Engineering Contradiction:
Improveenvironmental harmVSAvoidmanufacturing process complexity
Core Design Contradiction:
Object-affected harmful factorsVSDevice complexity

Solution Approach 1:

The patent applies preliminary action by pre-coating aggregates with bacterial cultures and nutrients before the biocalcification process. The bacteria are immobilized on the surface of sand and gravel particles in advance, and nutrient solutions are pre-applied to ensure optimal conditions for calcium carbonate precipitation. This preliminary preparation simplifies the overall manufacturing process by establishing the biological system before material assembly.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent introduces an intermediary substance - a carrier material or coating - that facilitates the attachment of bacteria to aggregates. This intermediary layer ensures uniform bacterial distribution and maintains contact between bacteria and substrate during the biocalcification process, simplifying the manufacturing by providing a reliable interface for the biological reaction without requiring complex process control.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Duration of action of stationary object

If biodegradable polymers are used as structural material, then biodegradability and environmental sustainability are improved, but resistance to high temperatures and mechanical strength are reduced

Engineering Contradiction:
ImprovebiodegradabilityVSAvoidthermal and mechanical strength
Core Design Contradiction:
Duration of action of stationary objectVSStrength

Solution Approach 1:

The patent creates composite materials by combining biodegradable polymer matrices with natural fibers (such as hemp, flax, or jute) and mineral aggregates bound by bacterial calcium carbonate. This composite structure leverages the biodegradability of polymers while compensating for their thermal and mechanical weaknesses through the reinforcement provided by fibers and the rigid calcium carbonate binding agent, achieving a balance between sustainability and performance.

Inventive Principle:
Principle #40Composite materials

Solution Approach 2:

The patent applies local quality by using biodegradable polymers in specific applications where temporary or low-load structures are needed, while employing bacterial-bound aggregate composites in areas requiring higher strength and thermal resistance. This localized material selection optimizes both biodegradability and mechanical performance according to the specific functional requirements of different structural components.

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method produces sustainable, non-toxic, and durable ecological bio-design products suitable for public health, enabling the creation of modular architectural and industrial materials that are environmentally friendly and suitable for various applications, including playgrounds and furniture, without the use of hazardous chemicals.

Implementation Method 1

these organisms have the ability to degrade urea. Due to this ability, they can produce the necessary ammonium to be used in their own metabolic activities

Methodology Applied
Scientific EffectUrea degradation: Hydrolysis

Implementation Method 2

studies have been carried out using microbiologically induced calcium carbonate (CaCO3) sedimentation

Methodology Applied
Scientific EffectMicrobiologically induced calcium carbonate sedimentation: Precipitation

Implementation Method 3

bio-mineralization of calcium carbonate

Methodology Applied
Scientific EffectBiomineralization: Crystallisation

Implementation Method 4

immobilization is carried out by means of attaching bacteria onto a biopolymer composite structure that is used as a support

Methodology Applied
Scientific EffectBacterial immobilization: Adsorption

Data Source

PatentEP3665135B1A bacterial biocalcification method for producing construction materials
Publication Date: 2023.04.12 DOKUZ EYLUL UNIVERSITESI REKTORLUGU
  • EP3665135B1 patent drawingFigure 1

AI summary

The invention is related to constructional, architectural, industrial materials that contain sand and fibers that are obtained by means of a bacterial biocalcification method, and products obtained by means of this method. Said construction material is formed into a composite structure that is manipulated by structures that have been obtained with biodegradable composites, using 3D modeling and printing technology.