Bacterial Consortium Composition for Methane Removal and Nitrogen Fixing

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Solution Overview

Problem

The climate crisis is exacerbated by methane (CH4), a potent greenhouse gas, and soil health has degraded due to farming practices, necessitating a solution to convert methane into a resource that nourishes soil and plants.

Innovation Solution

A bacterial consortium capable of consuming methane and fixing nitrogen at high rates, comprising various bacterial species and additives to enhance viability and efficiency.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If a bacterial consortium is designed to consume methane at high rates, then methane removal efficiency is improved, but the complexity of maintaining multiple bacterial species and their interactions increases

Engineering Contradiction:
Improvemethane consumption rateVSAvoidconsortium composition complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent combines multiple bacterial species with different functional capabilities (methane-consuming methanotrophs and nitrogen-fixing diazotrophs) into a single consortium system. This merging allows simultaneous achievement of high methane consumption rates and nitrogen fixation, resolving the contradiction by integrating multiple functions into one unified biological system rather than separate systems.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The bacterial consortium is designed to perform multiple functions simultaneously: consuming methane, fixing nitrogen, and providing nutrients to soils and plants. This multi-functionality allows the system to achieve high productivity in methane removal while also delivering agricultural benefits, effectively managing the complexity through functional integration rather than separate specialized systems.

Inventive Principle:
Principle #6Universality (Multi-functionality)

2Productivity

If multiple bacterial species are included in the consortium to enhance functionality, then nitrogen fixation capability is improved, but the difficulty of ensuring mutual non-interference between species increases

Engineering Contradiction:
Improvenitrogen fixation rateVSAvoidspecies interaction stability
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent employs compositional additives that enable bacteria to sense and respond to the presence of other species through quorum sensing mechanisms. This self-service capability allows the consortium to automatically regulate species interactions, ensuring that methanotrophs and diazotrophs coexist without harmful interference, thus maintaining reliability while achieving high nitrogen fixation rates.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The compositional additives facilitate feedback mechanisms where bacterial species monitor their environment and adjust their behavior accordingly. This feedback system ensures that species interactions remain stable and beneficial, with each species contributing its function (methane consumption or nitrogen fixation) while preventing negative interactions, thereby maintaining reliability across the diverse consortium.

Inventive Principle:
Principle #23Feedback

3Productivity

If the bacterial consortium is applied to improve soil health and provide nutrients, then agricultural productivity is improved, but the duration for which the benefits are sustained decreases

Engineering Contradiction:
Improvesoil nutrient availabilityVSAvoidviability duration
Core Design Contradiction:
ProductivityVSDuration of action of moving object

Solution Approach 1:

The patent formulates the bacterial consortium with compositional additives that ensure continuous viability and functional activity for extended periods. This continuity mechanism allows the consortium to maintain methane consumption and nitrogen fixation capabilities over time, sustaining soil health improvements and nutrient availability in agricultural systems without rapid degradation of effectiveness.

Inventive Principle:
Principle #20Continuity of useful action

Solution Approach 2:

The compositional additives modify physiological parameters of the bacterial consortium, enhancing their survival and functional persistence under varying environmental conditions. This parameter modification allows the bacteria to maintain high productivity in methane consumption and nitrogen fixation over extended durations, directly addressing the contradiction between immediate agricultural benefits and long-term sustainability.

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The consortium effectively consumes methane and fixes nitrogen, improving soil health and providing nutrients, with viability up to 12 months and enhanced nitrogen availability.

Implementation Method 1

consuming methane (CH4) at a rate greater than 13.5 umol/gDW per day

Methodology Applied
Scientific EffectOxidation: Oxidation

Implementation Method 2

fixing nitrogen (N2) at a rate greater than 2.0 umol/gDW per day

Methodology Applied
Scientific EffectNitrogen fixation:

Data Source

PatentUS20260077310A1Bacterial compositions for consuming methane and fixing nitrogen
Publication Date: 2026.03.19 WINDFALL BIO INC
  • US20260077310A1 patent drawing
  • US20260077310A1 patent drawing
  • US20260077310A1 patent drawing

AI summary

The disclosure provided herein describes a composition comprising a consortium of bacteria that is capable of consuming methane (CH4) and/or fixing nitrogen (N2) at a high rate.