Bacillus thuringiensis F201 Strain Insecticidal Efficacy

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Current Bacillus thuringiensis-based biological insecticides rely on recombinant DNA methods or artificial synthesis, which may not fully leverage natural endotoxin fragments for enhanced insecticidal efficacy, limiting their effectiveness against specific insect pests.

Innovation Solution

Identification and isolation of a Bacillus thuringiensis strain (B. thuringiensis F201) containing natural fragments of cry1Ab, cry1Ac, cry1D, and cry1E genes, along with a cry2A gene, which are used to create an insecticidal composition effective against Lepidoptera and other insect families, utilizing PCR selection and fermentation to enhance insecticidal activity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of manufacture

If recombinant DNA methods or artificial synthesis are used to produce Bacillus thuringiensis-based biological insecticides, then the production process can be standardized, but the insecticidal efficacy against specific insect pests is limited

Engineering Contradiction:

Improvestandardized production processVSAvoidinsecticidal efficacy

Core Design Contradiction:

Ease of manufactureVSReliability

Solution Approach 1:

The patent combines multiple endotoxin gene fragments (cry1Ab, cry1Ac, cry1D, cry1E, and cry2A) into a single Bacillus thuringiensis strain through natural coexistence rather than artificial recombination. This merging of multiple insecticidal genes within one strain achieves broader insecticidal spectrum and higher efficacy while maintaining natural compatibility among the genes

Inventive Principle:

Principle #5Merging (Combining)

Solution Approach 2:

The invention creates a composite endotoxin system by naturally co-expressing multiple different cry gene fragments in one bacterial strain. This composite approach integrates diverse insecticidal proteins that target different insect species, thereby enhancing overall insecticidal efficacy while maintaining standardized production capabilities

Inventive Principle:

Principle #40Composite materials

2Device complexity

If single gene fragment cloning is used in Bacillus thuringiensis, then the genetic engineering process is simple, but the insecticidal area is limited

Engineering Contradiction:

Improvegenetic engineering processVSAvoidinsecticidal area

Core Design Contradiction:

Device complexityVSAdaptability or versatility

Solution Approach 1:

The patent creates a multi-functional Bacillus thuringiensis strain that simultaneously expresses multiple endotoxin gene fragments targeting different insect orders (Lepidoptera, Diptera, Coleoptera). This single strain performs multiple insecticidal functions that would otherwise require separate strains or applications, greatly expanding the insecticidal area without proportionally increasing process complexity

Inventive Principle:

Principle #6Universality (Multi-functionality)

3Reliability

If multiple endotoxin genes are introduced through recombinant methods, then the insecticidal effect is improved, but the resistance of B. thuringiensis to worse environment is reduced

Engineering Contradiction:

Improveinsecticidal effectVSAvoidresistance to worse environment

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent utilizes the natural ability of Bacillus thuringiensis to harbor multiple plasmids and maintain multiple endotoxin genes through its own biological mechanisms rather than forced recombinant integration. The bacterium self-maintains these genes through natural plasmid replication and inheritance, preserving its natural environmental resistance while achieving enhanced insecticidal effect

Inventive Principle:

Principle #25Self-service

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The Bacillus thuringiensis F201 strain demonstrates significant insecticidal activity against targeted pests, such as Helicoverpa armigera, Trichoplusia ni, Maruca vitrata, and Plutella xylostella, with higher mortality rates compared to existing compositions, indicating improved insect control and broader spectrum efficacy.

Implementation Method 1

The insecticidal crystal protein produced from B. thuringiensis can inhibit the growth of some insect pests... Cry1 protein family has the insecticidal effect to Lepidoptera; Cry2 protein family shows the insecticidal effect to Lepidoptera and Diptera

Methodology Applied

Scientific EffectInsecticidal crystal protein toxicity:

Implementation Method 2

Methodology Applied

Scientific EffectPolymerase chain reaction:

Implementation Method 3

utilizing PCR selection and fermentation to enhance insecticidal activity

Methodology Applied

Scientific EffectMicrobial fermentation: Fermentation

Data Source

PatentUS8304225B2Bacillus thuringiensis strain for inhibiting insect pests

Publication Date: 2012.11.06 AGRI CHEM RES INST MINISTRY OF AGRI

AI summary

A novel bacterial strain of Bacillus thuringiensis for inhibiting insect pests is provided, wherein the Bacillus thuringiensis includes the gene fragments of cry1Ab, cry1Ac, cry1D, and cry1E.