Bacterial Composition Decomposing Trypsin via Protein Binding
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Solution Overview
Problem
The regulation of intraluminal proteins by microorganisms in the large intestine is not fully understood, particularly regarding the properties and functions of microorganisms related to the regulation of digestive enzymes.
Innovation Solution
A composition containing bacteria with 00502 or 00509 proteins or their homologues, which have trypsin-binding abilities, is used to decompose trypsin or TMPRSS2, utilizing a type IX secretion system and specific bacterial strains like Paraprevotella and Prevotella species, for therapeutic and diagnostic applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If bacteria with trypsin-binding ability are introduced to regulate protease activity, then the decomposition of trypsin and TMPRSS2 is improved, but the complexity of the composition increases
Solution Approach 1:
The patent uses bacteria as intermediary agents that naturally bind and decompose trypsin and TMPRSS2 through their cell surfaces and secreted proteins. This biological mediator approach achieves reliable protease decomposition without requiring complex chemical inhibition systems, thus resolving the contradiction between effectiveness and compositional simplicity
Solution Approach 2:
The bacteria possess inherent trypsin-binding abilities through their cell surface structures and secreted proteins, enabling them to self-regulate protease activity without external intervention. This self-service mechanism simplifies the overall composition while maintaining reliable decomposition function
2Reliability
If specific bacterial strains with 00502 or 00509 proteins are used, then the decomposition ability is improved, but the difficulty of detecting and measuring increases
Solution Approach 1:
The patent focuses on specific local characteristics of bacteria (presence of 00502 or 00509 proteins with trypsin-binding ability) rather than requiring comprehensive identification of all bacterial properties. This localized quality approach improves decomposition reliability while simplifying detection by concentrating on key functional markers
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition effectively regulates trypsin activity, providing a technique for treating diseases associated with trypsin or TMPRSS2, such as inflammatory bowel diseases and infectious conditions, by decomposing trypsin or TMPRSS2, thereby addressing the lack of understanding in microorganism regulation of digestive enzymes.
Implementation Method 1
bacteria that have 00502 protein, or a protein having 30% or higher sequence identity with an amino acid sequence of the 00502 protein and having a trypsin-binding ability; or bacteria that have 00509 protein, or a protein having 30% or higher sequence identity with an amino acid sequence of the 00509 protein and having a trypsin-binding ability
Data Source
AI summary
A composition for decomposition of trypsin or TMPRSS2 containing, as an active ingredient, bacteria that have 00502 protein, or a protein having 30% or higher sequence identity with an amino acid sequence of the 00502 protein and having a trypsin-binding ability; or bacteria that have 00509 protein, or a protein having 30% or higher sequence identity with an amino acid sequence of the 00509 protein and having a trypsin-binding ability.