Bacteriophage ΦCJ23 Antibacterial Composition for APEC Control

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Solution Overview

Problem

Current technologies lack effective solutions for preventing and treating infectious diseases caused by avian pathogenic Escherichia coli (APEC) in poultry, which poses significant economic damage to the poultry industry due to antibiotic resistance and residual antibiotic issues.

Innovation Solution

Development of a novel bacteriophage, ΦCJ23 (KCCM11365P), with specific bacteriocidal activity against APEC, and its incorporation into compositions such as antibiotics, feed additives, disinfectants, and cleaners to prevent and treat APEC infections.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If antibiotics are used to treat APEC infections, then bacterial growth is inhibited, but antibiotic resistance develops and antibiotics remain in meat

Engineering Contradiction:

Improveeffectiveness of treatmentVSAvoidantibiotic resistance and residual antibiotics

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent uses bacteriophage ΦCJ23 as an intermediary agent to treat APEC infections. Instead of using antibiotics directly, the bacteriophage infects and lyses APEC bacteria, eliminating the pathogen without leaving harmful residues or inducing resistance. This intermediary approach resolves the contradiction by providing effective treatment while avoiding the harmful effects of antibiotics.

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces the chemical mechanism of antibiotics with the biological mechanism of bacteriophage infection and lysis. The bacteriophage naturally targets and destroys APEC bacteria through its life cycle, substituting the chemical action of antibiotics with a biological process that does not create resistance or residues, thereby resolving the technical contradiction.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If conventional bacteriophages are used, then bacterial lysis is achieved, but they lack specificity or stability under processing conditions

Engineering Contradiction:

Improvebacteriocidal activityVSAvoidstability under acidic and heat stress

Core Design Contradiction:

ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent utilizes parameter changes in the bacteriophage's environment to enhance its stability. The bacteriophage ΦCJ23 maintains its structural integrity and infectivity across a range of temperatures and pH levels, allowing it to withstand processing conditions. This parameter stability enables the bacteriophage to remain effective both during application and under various storage and processing conditions.

Inventive Principle:

Principle #35Parameter changes

3Adaptability or versatility

If broad-spectrum antibiotics are used, then multiple bacterial infections are treated, but they affect beneficial bacteria and cause resistance

Engineering Contradiction:

Improvecoverage of bacterial infectionsVSAvoiddisruption of beneficial bacteria and resistance development

Core Design Contradiction:

Adaptability or versatilityVSObject-generated harmful factors

Solution Approach 1:

The patent applies the principle of local quality by using bacteriophage ΦCJ23 that specifically targets APEC bacteria with high host specificity. The bacteriophage infects only APEC and does not affect other beneficial bacteria in the poultry system. This localized action provides effective treatment for APEC infections while preserving the microbiome balance and preventing resistance development in other bacterial populations.

Inventive Principle:

Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

ΦCJ23 effectively targets APEC, offering resistance to acidic and heat stress, and can be used in various formulations to prevent and treat APEC-induced diseases in poultry, reducing antibiotic resistance and improving poultry health.

Implementation Method 1

bacteriophage is a specialized type of virus that infects and destroys only bacteria, and can self-replicate only inside host bacteria

Methodology Applied

Scientific EffectBacteriophage infection and lysis:

Implementation Method 2

confirmed that the bacteriophage had excellent acid resistance, heat resistance

Methodology Applied

Scientific EffectAcid resistance:

Implementation Method 3

confirmed that the bacteriophage had excellent acid resistance, heat resistance

Methodology Applied

Scientific EffectHeat resistance:

Data Source

PatentUS10704027B2Bacteriophage and antibacterial composition comprising the same

Publication Date: 2020.07.07 CJ CHEILJEDANG CORP

AI summary

Provided is a novel bacteriophage ΦCJ23 (KCCM11365P). In addition, the present invention relates to an antibacterial composition including the bacteriophage ΦCJ23 (KCCM11365P) as an active ingredient. Further, provided is a method of preventing and/or treating infectious diseases by avian pathogenic Escherichia coli(APEC) in birds using the bacteriophage ΦCJ23(KCCM11365P) or the antibacterial composition containing the bacteriophage ΦCJ23(KCCM11365P) as an active ingredient.