Base Sequencing Control With Parallel Reaction and Imaging
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Solution Overview
Problem
Current sequencing technologies face inefficiencies in sequencing time and cost due to the sequential nature of biochemical reactions and optical detection, which limits the overall sequencing efficiency and increases hardware costs.
Innovation Solution
The method involves dividing the reaction device into at least two components, where one component undergoes a biochemical reaction while the other is photographed, utilizing a fluid device and an optical device in parallel to perform biochemical reactions and imaging, thereby optimizing the sequencing process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If sequential biochemical reactions and optical detection are used, then the sequencing process is simple to implement, but the sequencing time is long and efficiency is low
Solution Approach 1:
The reaction device is divided into multiple reaction chambers (first reaction chamber, second reaction chamber, etc.), allowing different biochemical reactions to be performed simultaneously in different chambers. This segmentation enables parallel processing of sequencing steps, reducing total sequencing time while maintaining operational simplicity through modular design.
Solution Approach 2:
The patent performs preliminary actions by pre-preparing reaction mixtures and reagents in the reaction chambers before the actual sequencing begins. The fluid device is pre-configured with multiple reagent sources, allowing the sequencing process to start immediately without sequential preparation steps, thereby reducing overall sequencing time.
2Productivity
If multiple reaction chambers are used simultaneously, then sequencing efficiency is improved, but the device complexity increases
Solution Approach 1:
The fluid device is designed as a universal system that can service multiple reaction chambers through a single integrated fluid delivery mechanism. The same fluid device controls reagent delivery to all reaction chambers, eliminating the need for separate fluid delivery systems for each chamber, thus reducing overall device complexity while maintaining high productivity.
Solution Approach 2:
The patent introduces a control device as an intermediary that manages the coordination between multiple reaction chambers and the fluid device. This control device simplifies the system architecture by providing centralized management of reactions, fluid delivery timing, and data collection, reducing the operational complexity of managing multiple simultaneous reactions.
3Loss of time
If parallel biochemical reactions and imaging are performed, then sequencing time is reduced, but hardware costs increase
Solution Approach 1:
The patent merges the optical detection system with the reaction device by integrating the optical device directly into the reaction chamber structure. This integration allows simultaneous biochemical reactions and optical imaging within the same physical space, reducing the need for separate, standalone imaging hardware and thereby reducing overall hardware costs while achieving parallel processing.
Solution Approach 2:
The system uses optical copying/detection to capture information about the biochemical reactions without physically interfering with the reaction process. The optical device creates an optical copy or detection signal of the reaction state, allowing parallel imaging of multiple chambers without requiring physical sampling or manipulation of the actual reaction materials, thus reducing hardware complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces sequencing time and improves efficiency by effectively utilizing both optical and fluid devices, while also reducing overall sequencing costs.
Implementation Method 1
The detection module generally includes an optical detection module, a current detection module and a acid-base (pH) detection module. The sequencing platform based on the optical detection principle is used for sequence determination by analyzing variation in the optical signals collected from a sequencing biochemical reaction.
Data Source
AI summary
The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c. using the fluid device to perform the first biochemical reaction of the sample on the second component. The above-mentioned method can improve the efficiency of base sequence determination.