Beam Pattern Scanning Hologram Microscopy Without Moving the Sample
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Solution Overview
Problem
Conventional optical scanning hologram microscopes are limited by slow hologram acquisition speeds, particularly when imaging fluids like living bodies, due to mechanical movement of the object plate.
Innovation Solution
A flying-over beam pattern scanning hologram microscope device utilizing a spatial modulation scanner and translation stage, which modulates light beams to form high-resolution Fresnel zone patterns and projects them onto an object plane, combined with a light collection unit to detect reflected or fluoresced beams, enabling high-speed imaging.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If mechanical movement of object plate is used to scan the object, then the scanning hologram microscope can acquire holograms, but the hologram acquisition speed becomes slow
Solution Approach 1:
The patent replaces the mechanical object plate movement system with an optical beam scanning system. A spatial light modulator (SLM) modulates the phase of light to create a moving beam pattern that scans across the object without mechanical movement. This substitution of mechanical scanning with optical scanning dramatically increases the hologram acquisition speed, enabling real-time imaging of dynamic samples such as living cells and fluids.
2Reliability
If mechanical method is used to move the object plate, then the object can be scanned, but it becomes difficult to acquire holograms of fluid samples
Solution Approach 1:
The patent eliminates mechanical contact with the sample by replacing the moving object plate with a stationary sample stage and a moving optical beam pattern. The SLM generates phase-modulated light that creates a scanning beam pattern, allowing holograms to be acquired from fluid samples without mechanical disturbance. This enables stable, high-speed imaging of dynamic fluid samples such as living cells and biological fluids.
3Measurement precision
If conventional optical scanning method is used, then holograms can be acquired, but the resolution and scanning speed cannot be simultaneously optimized
Solution Approach 1:
The patent employs dynamic phase modulation of the optical beam using a spatial light modulator (SLM). The SLM rapidly changes the phase distribution of light to create a moving beam pattern that scans across the object. This dynamic optical scanning enables simultaneous achievement of high resolution (through precise beam control) and high scanning speed (through rapid phase modulation), overcoming the trade-off inherent in conventional mechanical scanning systems.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device achieves high-resolution scanning holograms at increased speeds by forming a flying-over scan beam pattern on the object, allowing for rapid imaging of fluid samples.
Implementation Method 1
allows the first and second spherical waves to interfere with each other to form a scan beam
Implementation Method 2
a spatial modulation scanner for controlling the incident scan beam in a horizontal direction to be transferred to a projection unit
Implementation Method 3
forming the formed beam pattern into a high-resolution Fresnel zone pattern on an object plane through a objective lens
Implementation Method 4
a light collection unit which detects a beam that has passed through the objective lens again after fluorescing or is reflected from an object
Implementation Method 5
a light collection unit which detects a beam that has passed through the objective lens again after fluorescing or is reflected from an object
Data Source
AI summary
A flying-over beam pattern scanning hologram microscope device includes: a scan beam generation unit which converts of a first beam and a second beam to a first spherical wave; a scanning unit, which includes a spatial modulation scanner for controlling the scan beam in the horizontal direction, and a translation stage for moving an object in the vertical direction at the rear end of the projection unit; the projection unit projecting the scan beam onto an object plane; and a light collection unit which detects a beam that has passed through the objective lens again after being reflected or fluoresced from the object.