Rhodotorula Toruloides Bel 1 Beta-Carotene Fermentation and Green Extraction
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
There is a lack of knowledge on the biosynthesis method of beta carotene from Rhodotorula toruloides Bel 1 strain, including the composition of culture medium, culture conditions, and isolation and purification methods, which limits its application in the food industry and animal feed.
Innovation Solution
A method is developed for producing beta carotene using the Rhodotorula toruloides Bel 1 strain, involving a specific culture medium composition, cultivation conditions, and extraction process using environmentally friendly solvents, which includes glycerol, vegetable fat, and yeast extract, followed by biosynthesis, separation, and extraction using a solvent mixture under nitrogen atmosphere.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional organic solvent extraction methods are used for carotenoid extraction, then extraction efficiency is improved, but environmental pollution and processing costs increase
Solution Approach 1:
The patent changes the chemical parameters of the extraction system by replacing traditional organic solvents with a deep eutectic solvent system composed of choline chloride, ethylene glycol, and water. This parameter change maintains extraction efficiency while eliminating the harmful environmental effects of volatile organic solvents, directly resolving the contradiction between productivity and environmental harm.
Solution Approach 2:
The patent employs a composite solvent system combining multiple components (choline chloride, ethylene glycol, and water) to create a deep eutectic solvent with properties superior to individual components. This composite approach achieves both high extraction efficiency and environmental sustainability, simultaneously addressing both sides of the contradiction.
2Ease of manufacture
If chemical synthesis methods are used for carotenoid production, then production cost is reduced, but product health value and market demand decrease
Solution Approach 1:
The patent utilizes yeast cells as self-service bioreactors that naturally synthesize beta-carotene through their metabolic pathways. The organism serves itself to produce the desired compound, eliminating the need for complex chemical synthesis processes while maintaining product quality and health value, thus resolving the contradiction between manufacturing ease and product reliability.
Solution Approach 2:
The patent introduces a biological intermediary (yeast strain) that mediates the production process between simple fermentation conditions and complex chemical synthesis. This intermediary enables cost-effective production through biological pathways while ensuring the natural health properties of carotenoids are preserved, balancing both sides of the contradiction.
3Productivity
If new yeast strain isolation and cultivation methods are developed, then beta carotene production capability is improved, but research and development time and resources increase
Solution Approach 1:
The patent performs preliminary actions by pre-optimizing the yeast strain selection, culture medium composition, and fermentation parameters before scale-up production. The detailed preliminary characterization of the strain's physiological properties and optimal growth conditions accelerates the transition from laboratory to industrial production, reducing overall development time while maintaining high productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enables enhanced beta carotene synthesis with high concentration and economic benefits through complete regeneration of extractives, reducing environmental impact and processing costs.
Implementation Method 1
Biosynthesis of beta carotene from the culture medium in the previous step at 25-30°C and duration 72-115 hours with constant stirring
Implementation Method 2
separation of yeast biomass in a centrifuge at 4000 - 6500 min -1
Data Source
AI summary
The invention relates to a newly isolated yeast strain Rhodotorula toruloides Bel 1 and a method for producing beta carotene developed therefrom, which will find application in the food industry and in particular in the feeding of farm animals and fish. The strain assimilates glucose, sorbose, ribose, sucrose, melibiose, raffinose, inulin, pectin, glycerol, ribitol, mannitol, citric acid, and does not assimilate cellobiose, lactose, starch, erythritol, galactitol, inositol, glucosamine and tartaric acid. Of nitrogenous sources it assimilates inorganic ammonium and nitrate salts, amino acids, does not assimilate urea. Strain grows on nutrient media without the presence of vitamins. The optimum temperature for its development is in the range of 25-30°C, with temperatures above 35°C slowing its development considerably. A medium formulation has been developed which contains glycerol, vegetable fats, yeast extract, KH2PO4, NH2(SO4) and MgSO4. After sterilization and cooling, the culture medium was inoculated with a liquid culture of Rhodotorula toruloides Bel 1 strain. The strain has a pronounced capacity for biomass synthesis with a high concentration of carotenes.