Novel Beta-Galactosidase Enzyme for Low-Lactose Milk Production
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Solution Overview
Problem
The production of enzyme preparations suitable for industrial applications, such as low-lactose milks and galacto-oligosaccharides, is limited due to unclear protein chemical correlations and molecular biological characteristics of β-galactosidases derived from Bacillus circulans.
Innovation Solution
A novel β-galactosidase with a molecular weight of 195 kDa, referred to as β-Gal1, is isolated and its gene is cloned, revealing a distinct amino acid sequence and high transgalactosylation activity, allowing for the production of recombinant enzymes with specific properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If β-galactosidase from Bacillus circulans is used for industrial applications, then transgalactosylation activity is improved, but protein chemical correlation and molecular biological characteristics are unclear
Solution Approach 1:
The patent segments the complex β-galactosidase preparation into distinct molecular forms with molecular weights of 195 kDa, 160 kDa, and 86 kDa. By separating and characterizing each form individually through purification and gene cloning, the patent establishes clear protein chemical correlations and molecular biological characteristics for each segment, resolving the information loss about the enzyme's composition and properties.
2Reliability
If multiple β-galactosidase forms are present, then enzyme activity is improved, but enzyme preparation suitability for industrial applications is limited
Solution Approach 1:
The patent extracts and isolates specific β-galactosidase forms (195 kDa, 160 kDa, and 86 kDa) from the complex mixture through systematic purification. By taking out each form separately and characterizing them individually, the patent enables selective use of appropriate enzyme forms for specific industrial applications, improving ease of manufacture while maintaining high enzyme activity.
Solution Approach 2:
The patent assigns different functional properties to different molecular forms of β-galactosidase. The 195 kDa form exhibits high transgalactosylation activity, while other forms have different activity profiles. By matching specific enzyme forms to specific application requirements, the patent optimizes local quality characteristics for each industrial use case.
3Adaptability or versatility
If gene cloning is performed to establish molecular biological characteristics, then production of recombinant enzymes is enabled, but complexity of research and development increases
Solution Approach 1:
The patent performs preliminary gene cloning and characterization work to establish the molecular biological characteristics of β-galactosidase before industrial production. By completing the gene sequencing, expression system development, and recombinant protein production methodology in advance, the patent creates a foundation that simplifies future large-scale production while maintaining adaptability for different application needs.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The novel β-galactosidase and its recombinant forms demonstrate enhanced transgalactosylation activity and hydrolyzing capabilities, enabling effective production of low-lactose milks and galacto-oligosaccharides, addressing the limitations of previous enzyme preparations.
Implementation Method 1
Beta-galactosidase (EC3.2.1.23) is an enzyme that hydrolyzes the β-D-galactoside bond to release D-galactose
Implementation Method 2
hydrolyzes the β-D-galactoside bond
Implementation Method 3
β-galactosidase has an ability to transfer the galactoside bond, and a method to prepare galacto-oligosaccharides (oligosaccharides with galactose residues) using this ability is known
Data Source
AI summary
Disclosed is a novel β-galactosidase. Specifically disclosed are a β-galactosidase derived from Bacillus circulans and a gene for the β-galactosidase. The β-galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.