Bi-telecentric Imaging System for Dual-Mode Macro-Micro Scanning

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Solution Overview

Problem

Current imaging systems lack the capability to seamlessly switch between macroscopic and microscopic imaging modes using the same optical system, limiting their ability to provide detailed quantitative measurements and efficient imaging of samples.

Innovation Solution

A dual-mode imaging system utilizing a bi-telecentric imaging system that enables controllable switching between macroscopic and microscopic imaging modes, allowing for large area scanning and high magnification zooming using the same bi-telecentric line-scanning imager, with integrated laser combining modules and relay optics for efficient illumination and detection.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Device complexity

If a single optical imaging system is used for both macroscopic and microscopic imaging, then device complexity is reduced and resource efficiency is improved, but the system must accommodate multiple imaging modes which increases operational complexity

Engineering Contradiction:
Improvenumber of imaging systemsVSAvoidswitching between imaging modes
Core Design Contradiction:
Device complexityVSEase of operation

Solution Approach 1:

The patent implements a single optical imaging system that can operate in both macroscopic and microscopic imaging modes by incorporating mode-switching capability. The system uses a stage that can be positioned at different locations (first location for macroscopic, second location for microscopic) and relay optics that can redirect illumination beams through objective lenses. This multi-functional design eliminates the need for separate imaging systems while maintaining both imaging capabilities.

Inventive Principle:
Principle #6Universality (Multi-functionality)

2Device complexity

If macroscopic imaging is performed with a standard imaging system, then the system structure is simple, but measurement precision and quantitative accuracy are insufficient

Engineering Contradiction:
Improveimaging system structureVSAvoidquantitative measurement accuracy
Core Design Contradiction:
Device complexityVSMeasurement precision

Solution Approach 1:

The patent employs a dynamic stage positioning system that can be moved between different locations to enable both macroscopic and microscopic imaging modes. The stage position is dynamically adjusted based on the imaging mode requirement, and the system includes control mechanisms to maintain precise positioning. This dynamic adaptability allows the system to achieve high measurement precision in macroscopic mode by positioning the stage at the first location and using the illumination beam directly, while also enabling microscopic mode when needed.

Inventive Principle:
Principle #15Dynamics

3Reliability

If separate imaging systems are used for macroscopic and microscopic imaging, then each system can be optimized for its specific function, but loss of time and resources increases due to having multiple instruments

Engineering Contradiction:
Improvefunction-specific optimizationVSAvoidtime to switch between instruments
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent combines macroscopic and microscopic imaging capabilities into a single integrated system. The same optical imaging system, illumination source, and detector are used for both imaging modes by adjusting the stage position and configuring the relay optics. This merging eliminates the need to physically move samples between separate instruments, saving time and resources while maintaining the functional optimization of both imaging modes through dedicated optical paths and control mechanisms.

Inventive Principle:
Principle #5Merging (Combining)

4Measurement precision

If high magnification is achieved through additional optics, then microscopic imaging capability is improved, but device complexity and cost increase

Engineering Contradiction:
Improvemicroscopic imaging resolutionVSAvoidoptical system components
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent uses relay optics as an intermediary component to enable microscopic imaging mode. The relay optics are configured to receive the illumination beam and redirect it through an objective lens onto the sample, allowing high magnification without requiring a completely separate microscope system. This intermediary optical path provides the necessary magnification capability while integrating smoothly with the existing optical imaging system, minimizing additional complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables fast, sensitive, and quantitative imaging with direct macroscopic scanning and high-resolution microscopic imaging, saving time and resources by using the same instrument for both modes, suitable for various scientific applications including fluorescence imaging.

Implementation Method 1

a bi-telecentric imaging system adapted to receive the emission beam from the sample and project the emission beam onto a detector array

Methodology Applied
Scientific EffectTelecentric optics:

Implementation Method 2

relay optics that are configured to receive and redirect the illumination beam through an objective lens when the preparation is in a certain location relative to the imaging system

Methodology Applied
Scientific EffectOptical focusing: Lens

Implementation Method 3

a CCD that acquires a macro image of the observation region by capturing transmitted light from the observation region

Methodology Applied
Scientific EffectLight detection: Photoelectric Effect

Implementation Method 4

a light source that radiates excitation light onto the biological specimen and a micro-image acquisition unit that acquires a micro image of the biological specimen by detecting fluorescence generated at a position in the biological specimen irradiated by the excitation light

Methodology Applied
Scientific EffectFluorescence: Fluorescence

Data Source

PatentEP3918404B1Macro-micro telecentric scanning systems and methods
Publication Date: 2024.12.25 LI COR BIOTECH LLC
  • EP3918404B1 patent drawingFigure 1
  • EP3918404B1 patent drawingFigure 2
  • EP3918404B1 patent drawingFigure 3A

AI summary

Dual mode imaging systems and methods for macroscopic and microscopic imaging using the same optical imaging system (OIS). The various embodiments enable controllable and/or automated switching between macroscopic imaging and microscopic imaging modes. A dual mode imaging system includes a sample platform movable relative to an OIS between first and second locations, and a light source subsystem configured to generate and project an illumination beam onto a focal plane. When in the first location, the sample platform coincides with the focal plane, and the OIS receives light from the sample platform along a first detection light path. When in the second location, the illumination beam interacts with relay optics and impinges on the sample platform through an objective lens, and the light from the sample platform is directed back through the objective lens and relay optics to the OIS via the first detection path.