Bicarbonate-Based Release Reagent for Analyte Extraction
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Solution Overview
Problem
Current in vitro diagnostic methods face challenges in efficiently releasing analytes from samples, particularly due to interference from complex sample matrices, which affects downstream applications like bead enrichment in mass spectrometry, and require manual handling of chemically unstable reagents, leading to errors and short shelf-life issues.
Innovation Solution
An in vitro method involving a chaotropic agent, an organic solvent, a detergent, and at least one agent providing bicarbonate ions is used to release analytes from samples, improving extraction efficiency and reducing interference with downstream applications while stabilizing reagents for longer shelf-life.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If high concentrations of chaotropic agents are used to release analytes from sample matrix, then analyte release efficiency is improved, but ion suppression in mass spectrometry occurs
Solution Approach 1:
The patent changes the chemical parameters of the release reagent by using bicarbonate ions instead of high concentrations of chaotropic agents. This parameter change maintains analyte release efficiency while eliminating ion suppression in mass spectrometry, as bicarbonate ions do not cause the same suppression effects as chaotropic agents.
Solution Approach 2:
The patent introduces bicarbonate ions as an intermediary substance that mediates the release of analytes from the sample matrix without causing harmful side effects. The bicarbonate ions act as a gentler alternative to chaotropic agents, achieving the same release function without ion suppression.
2Productivity
If chemically unstable reagents are used for analyte release, then extraction efficiency is improved, but shelf-life is reduced and manual handling errors increase
Solution Approach 1:
The patent employs a stable reagent formulation that can be stored for extended periods without degradation. This stable formulation eliminates the need for frequent reagent replacement and manual reconstitution, improving reliability while maintaining extraction efficiency through optimized bicarbonate ion concentration and composition.
Solution Approach 2:
The patent prepares a stable reagent formulation in advance that maintains its effectiveness over time. The bicarbonate-based composition is designed to be stable during storage, allowing preliminary preparation without loss of extraction efficiency, thus eliminating the need for manual handling of unstable reagents.
3Productivity
If organic solvents are used for analyte release, then matrix removal is improved, but compatibility with downstream bead enrichment applications is reduced
Solution Approach 1:
The patent changes the solvent system from organic solvents to an aqueous bicarbonate-based solution. This parameter change maintains matrix removal capability while ensuring compatibility with downstream bead enrichment applications, as aqueous solutions do not interfere with bead functionality in mass spectrometry workflows.
Solution Approach 2:
The bicarbonate-based release reagent is designed to be universally compatible with multiple downstream applications, including bead enrichment and mass spectrometry. The aqueous formulation serves multiple functions: releasing analytes, removing matrix, and maintaining compatibility with subsequent analytical steps without requiring additional adaptation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high extraction efficiency for steroid hormones, oligopeptides, and vitamins, reducing the need for high chaotropic agent concentrations and avoiding ion suppression in mass spectrometry, with stable reagents that maintain effectiveness over time.
Implementation Method 1
contacting said sample with (i) a chaotropic agent
Implementation Method 2
contacting said sample with (ii) an organic solvent
Implementation Method 3
contacting said sample with (iii) a detergent
Data Source
Figure 1
Figure 2
Figure 3a~3b
AI summary
The present invention relates to an in vitro method for releasing analytes from a sample comprising contacting said sample with (i) a chaotropic agent, (ii) an organic solvent, (iii) a detergent, and (iv) at least one agent providing bicarbonate ions. The present invention further relates to a release agent for releasing analytes from a sample comprising the aforesaid compounds. Moreover, the present invention relates to uses, kits, methods and devices related to the method and the release agent of the present invention.