Bifunctional Pyr Regulator Variant for IMP Yield
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Solution Overview
Problem
Current methods for increasing IMP production in microorganisms, such as those involving the bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase, do not effectively enhance yield to meet growing demand.
Innovation Solution
A novel bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase variant is developed, where proline at position 108 is substituted with serine, and a Corynebacterium stationis strain is engineered to express this variant, resulting in increased IMP production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the expression of gene encoding bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase is increased, then IMP biosynthesis capability is improved, but the IMP production yield does not effectively increase to meet growing demand
Solution Approach 1:
The patent applies parameter changes by substituting proline at position 108 with serine in the bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase protein sequence. This amino acid substitution modifies the enzyme's catalytic properties, transforming it from a state where increased expression does not effectively enhance IMP production to a state where the enzyme achieves significantly improved catalytic efficiency and IMP production yield.
2Productivity
If target substance-specific approach is used to increase gene expression, then IMP biosynthesis is enhanced, but further improvement in production ability requires additional studies
Solution Approach 1:
Rather than implementing complex multi-gene expression systems or multiple genetic modifications, the patent achieves enhanced IMP producing ability through a single, targeted amino acid substitution (proline to serine at position 108) in the bifunctional enzyme. This simplifies the genetic modification approach while effectively increasing IMP production capability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The engineered strain achieves a higher yield of IMP compared to existing microorganisms with unmodified polypeptides, demonstrating enhanced production capabilities.
Implementation Method 1
bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase variant... in which proline, which is an amino acid corresponding to position 108 of an amino acid sequence of SEQ ID NO: 3, is substituted with serine
Data Source
AI summary
The present disclosure relates to a novel bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase variant, a Corynebacterium stationis strain comprising the variant, and a method for producing IMP using the strain.