Bimodal Chromatography Media for Reduced Back Pressure
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Chromatography media with desired binding capacity and efficiency often restrict high flow rates due to particle size and rigidity, leading to high back pressures, and increasing bead size to reduce back pressure results in lower resolution and dynamic binding capacity.
Innovation Solution
A method involving a mixture of small particles with a functionalized outer shell and a larger, non-functionalized or partially functionalized core, where the ratio of large to small beads is greater than 1.2, and the volume ratio is between 0.05-0.9, to reduce flow resistance while maintaining adsorption kinetics and elution efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stress or pressure
If larger size beads are used to reduce back pressures, then pressure drop decreases, but resolution and dynamic binding capacities are reduced
Solution Approach 1:
The chromatography media is segmented into two distinct particle size populations: small particles (10-50 μm) that provide high resolution and binding capacity, and large particles (50-200 μm) that reduce back pressure. This segmentation allows each size fraction to fulfill its specific function optimally within the packed bed
Solution Approach 2:
Different regions of the packed bed are assigned different particle sizes based on local functional requirements. Small particles are placed in regions where high resolution and binding capacity are critical, while large particles are positioned in regions where flow throughput and pressure management are prioritized
2Stress or pressure
If larger size beads are used to reduce back pressures, then flow resistance decreases, but dynamic binding capacity is reduced
Solution Approach 1:
The chromatography media is segmented into two distinct particle size populations: small particles (10-50 μm) that provide high resolution and binding capacity, and large particles (50-200 μm) that reduce back pressure. This segmentation allows each size fraction to fulfill its specific function optimally within the packed bed
Solution Approach 2:
The invention merges two particle size populations with complementary properties into a single chromatography media system. The small particles contribute binding capacity while the large particles contribute low flow resistance, and their combined effect achieves both high dynamic binding capacity and reduced back pressure simultaneously
3Manufacturing precision
If small particles are used to maintain resolution and binding capacity, then chromatographic performance is improved, but back pressure increases
Solution Approach 1:
The chromatography media is segmented into two distinct particle size populations: small particles (10-50 μm) that provide high resolution and binding capacity, and large particles (50-200 μm) that reduce back pressure. This segmentation allows each size fraction to fulfill its specific function optimally within the packed bed
Solution Approach 2:
The invention changes the particle size parameter from a uniform distribution to a bimodal distribution with specific size ranges. This parameter change transforms the pressure-flow characteristics while maintaining resolution, as the large particles provide flow channels that reduce back pressure without affecting the resolution provided by small particles
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach optimizes column packing for reduced back pressure and preserved chromatographic performance, allowing higher sample loads without sacrificing resolution or binding capacity, thus enhancing pressure-flow characteristics.
Implementation Method 1
decrease the flow resistance in the packed bed
Implementation Method 2
small particles with the same type of functionalization (ligand) in the shell
Implementation Method 3
The nature of the ligands is not limiting to the broad aspect of the present invention. Thus, in one embodiment of the present separation matrix, the ligands are selected from the group consisting of anion exchange ligands; cation exchange ligands; hydrophobic interaction chromatography (HIC) ligands; reversed phase chromatography (RPC) ligands; immobilised metal affinity chromatography (IMAC) ligands; thiophilic ligands; affinity ligands
Data Source
AI summary
The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalization as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [D50V for large particles/D50V for small particles]>1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beads/Total volume beads] is in the range 0.05-0.9.