Multi-Channel Bio-Separation Cartridge with Integrated Fluorescence Detection
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Solution Overview
Problem
Current bio-separation tools, particularly slab gel electrophoresis, are labor-intensive, costly, and have low throughput, while capillary electrophoresis systems are expensive due to complex optical detection mechanisms, making them inaccessible to many laboratories.
Innovation Solution
A multi-channel capillary electrophoresis system with a simplified, low-cost design that includes a cartridge-based bio-separation system with integrated separation channels and dual fiber optic fluorescence detection, allowing for automated bio-separation without the need for fine optical alignment, and using interchangeable cartridges with separate reagent reservoirs for each channel.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If slab gel electrophoresis is used for bio-separation, then the system is simple and accessible, but the process is labor-intensive with low throughput and high cost per sample
Solution Approach 1:
The invention divides the bio-separation process into discrete, automated steps within a microfluidic chip architecture. The chip is segmented into distinct functional zones (sample injection, separation, detection) that operate automatically, eliminating manual labor while maintaining simplicity. This segmentation enables high-throughput analysis without increasing operational complexity.
2Measurement precision
If capillary electrophoresis with laser-induced fluorescence detection is used, then sensitivity and resolution are improved, but the system cost increases significantly
Solution Approach 1:
The invention extracts the complex optical detection mechanism from the overall system and replaces it with a simplified fluorescence detection approach integrated into the microfluidic chip. By using fluorescently labeled samples and simple optical detectors rather than laser-induced fluorescence, the system achieves comparable sensitivity without the mechanical complexity and high cost of traditional CE-LIF systems.
Solution Approach 2:
The invention employs disposable microfluidic chips that integrate separation and detection functions. These single-use chips eliminate the need for expensive, complex optical alignment mechanisms and maintenance, providing cost-effective high-sensitivity detection. The disposable nature ensures consistent performance without the need for recalibration or complex optical systems.
3Manufacturing precision
If traditional CE systems are used, then high resolution bio-separation is achieved, but the system requires fine optical alignment and has high maintenance requirements
Solution Approach 1:
The invention merges the separation channel and detection zone into a single integrated microfluidic chip structure. This integration eliminates the need for separate optical alignment components and reduces maintenance requirements. The chip's fixed geometry ensures consistent separation resolution without requiring manual alignment or complex mechanical adjustments.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system provides high-throughput, efficient, and sensitive bio-analysis with reduced operational costs, enabling rapid analysis of multiple samples with improved resolution and sensitivity, making it more accessible to laboratories.
Implementation Method 1
a capillary separation channel (12) formed within the cartridge body, wherein the capillary separation channel has a first end and a second end
Implementation Method 2
slab gel based electrophoresis technologies, which have routinely been used for bio-analysis of bio-molecules
Implementation Method 3
dual fiber optic fluorescence detection, allowing for automated bio-separation without the need for fine optical alignment
Implementation Method 4
CE with laser-induced fluorescence (LIF) is one of the most powerful analytical tools for rapid, high sensitivity and high-resolution bio-analysis
Data Source
AI summary
A multi-channel bio-separation system configured to utilize a cartridge that has a individual, separate integrated reagent (i.e., a separation buffer) reservoir dedicated for each separation channel. The multiple channels may have different characteristics, such as different separation medium of different chemistries, different separation length, different channel sizes and internal coatings. In one embodiment, the cartridge does not include integrated detection optics. Not all channels need to be operative. One or more of the channels in the cartridge may be “dummy channels” that are not operative (e.g., not provided with a capillary tube). A capillary tube may be routed between the reservoir/electrode (anode) of one channel to an electrode (cathode) in another channel, thus allowing a longer length of capillary tube to be used to define a longer separation channel to improve resolution.