Biochemical Analyzer Using Single-Band Filters and Crosstalk Matrix
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Solution Overview
Problem
Existing biochemical analyzers face challenges in accurately determining concentrations of fluorescent substances in solutions due to the need for complex filter mechanisms or multiple-band filters, which result in large dimensions, reduced precision, and crosstalk interference, making it difficult to produce portable and efficient analyzers.
Innovation Solution
The analyzer employs a microreactor with separate excitation and detection filters for each type of fluorophore, using RGB image sensors to isolate and process signals from each fluorophore, eliminating the need for filter replacement and enhancing precision by using single-band filters and crosstalk matrix calculations to determine concentrations.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If multiple-band filters are used to eliminate the need for filter replacement mechanisms, then device complexity is reduced, but measurement precision deteriorates due to crosstalk and poor selectivity
Solution Approach 1:
The patent divides the detection system into multiple independent detection channels, each equipped with its own single-band filter optimized for a specific fluorophore. This segmentation allows each channel to independently and selectively detect specific fluorophores without interference, eliminating crosstalk while maintaining high precision. The system processes signals from multiple channels simultaneously to achieve multi-fluorophore detection.
2Adaptability or versatility
If filter replacement mechanisms are implemented to analyze multiple fluorophores, then adaptability is improved, but device complexity and dimensions increase
Solution Approach 1:
The patent merges multiple detection channels into a single integrated detection system. Each channel includes a photodetector with a specific single-band filter, and all channels share common components such as the microfluidic chip, light source, and signal processing electronics. This merging approach enables multi-fluorophore analysis capability while maintaining a compact structure without requiring filter replacement mechanisms.
3Ease of manufacture
If multiple-band filters are used to reduce device complexity, then ease of manufacture is improved, but manufacturing precision requirements worsen due to the need for precise spectral separation
Solution Approach 1:
The patent applies local quality by assigning each detection channel a dedicated single-band filter with optimized spectral characteristics tailored to its specific fluorophore. Instead of using a generic multiple-band filter across all channels, each channel has customized filter properties (center wavelength, bandwidth) matched to the excitation and emission spectra of the target fluorophore. This localized optimization ensures high spectral selectivity and eliminates crosstalk.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for accurate and precise determination of substance concentrations without filter replacement, reducing mechanical complexity and enabling the design of compact, portable analyzers with improved precision and reduced interference.
Implementation Method 1
The substrate is exposed to a light source having an appropriate spectrum of emission such as to excite the fluorophores. In turn, the excited fluorophores emit a secondary radiation at an emission wavelength greater than the peak of the excitation spectrum.
Implementation Method 2
The light emitted by the fluorophores is collected and detected by an optical sensor.
Data Source
AI summary
An analyzer for biochemical analysis includes a seat for receiving a recipient. A first light source and a second light source illuminate the recipient with a luminous radiation, respectively, in a first excitation band and in a second excitation band, including a first excitation wavelength and a second excitation wavelength of fluorophores of a first type and of a second type. A first image sensor and a second image sensor are oriented so as to receive light emitted by fluorophores contained in the recipient and are, respectively, provided with a first detection filter and a second detection filter, having, respectively, a first detection passband and a second detection passband, including, respectively, a first emission wavelength and a second emission wavelength of the fluorophores of the first type and of the second type.