Biochip Geometric Structures for Nucleic Acid Sequencing Stability
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Solution Overview
Problem
Existing biochips face challenges in achieving stable and accurate nucleic acid sequencing due to inadequate design of micro/nano structures on the chip surface, which affects sequencing indicators and parameters such as clonal cluster stability, signal intensity, and sequencing quality.
Innovation Solution
A patterned chip with regularly arranged geometric structures on its surface, where the geometric structures have specific dimensions (height of 10 nm to 100 µm, maximum dimension of 10 nm to 100 µm, and distance between structures of 10 nm to 100 µm) to improve sequencing stability and accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If geometric structures with specific dimensions are designed on the chip surface, then sequencing stability and accuracy are improved, but device complexity increases
Solution Approach 1:
The patent applies parameter changes by optimizing the geometric dimensions of structures on the chip surface. Specifically, it controls the height of geometric structures to be 10-100 μm, the maximum dimension to be 10-100 μm, and the distance between adjacent structures to be 10-100 μm. These precise parameter specifications improve sequencing stability and accuracy without requiring fundamental changes to the chip architecture.
Solution Approach 2:
The patent implements local quality by creating patterned arrangements of geometric structures at specific locations on the chip surface. The regular arrangement with controlled spacing creates localized detection sites that enhance sequencing performance in specific regions, thereby improving overall reliability through targeted structural optimization.
2Measurement precision
If geometric structures with specific dimensions are designed on the chip surface, then sequencing accuracy is improved, but manufacturing precision requirements increase
Solution Approach 1:
The patent specifies a relatively wide parameter range for geometric structure dimensions (10-100 μm for height and maximum dimension, and 10-100 μm for spacing). This range is wide enough to accommodate normal manufacturing variations while still achieving the desired sequencing accuracy, thereby reducing the stringency of manufacturing precision requirements.
3Productivity
If regularly arranged geometric structures are configured on the chip surface, then detection throughput is improved, but device complexity increases
Solution Approach 1:
The patent divides the chip surface into multiple regularly arranged geometric structures, each serving as an independent detection site. This segmentation allows parallel processing of multiple samples simultaneously, thereby increasing detection throughput. The regular arrangement pattern facilitates systematic organization of detection sites across the chip surface.
Solution Approach 2:
The patent utilizes two-dimensional regular arrangements of geometric structures on the chip surface, transitioning from single-point detection to multi-point parallel detection. This spatial dimensionality expansion enables simultaneous detection of multiple nucleic acid molecules, significantly improving throughput without requiring sequential processing.
4Quantity of substance
If regularly arranged geometric structures are configured on the chip surface, then arrangement density is improved, but manufacturing complexity increases
Solution Approach 1:
The patent specifies that the distance between adjacent geometric structures should be 10-100 μm, which provides sufficient spacing to accommodate manufacturing tolerances while achieving high arrangement density. This parameter optimization allows for increased number of detection sites without making the fabrication process excessively complex.
Solution Approach 2:
The patent employs regular arrangement patterns of geometric structures that create consistent local environments for nucleic acid detection. This regularity simplifies the manufacturing process by enabling standardized fabrication techniques to be applied across the entire chip surface, rather than requiring complex variable patterning.
Data Source
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AI summary
The present application discloses a chip, comprising a chip substrate provided with regularly arranged geometric structures on at least one surface. The geometric structure has a height of 10 nm to 100 µm in a direction perpendicular to the surface of the chip and a maximum dimension of 10 nm to 100 µm in a direction parallel to the surface of the chip. The distance between adjacent geometric structures is 10 nm to 100 µm. By forming the regularly arranged geometric structures on the chip substrate, the present application allows a controllable arrangement of biomolecule detection sites and a high density, and can thus improve the detection throughput and detection cost-efficiency. The dimensional parameters of the biochip geometric structure provided herein can improve the sequencing quality, and feature high repeatability, good stability, and resistance to multiple cycles of reagent scouring.