Biological Analysis Chip Assembly for Precise Porous Test Zones

The patent replaces chemical ink printing processes and thermal treatments with a mechanical punching system. A punch tool with a precision-machined cavity is pressed against the matrix material to directly form analysis zones with controlled geometry. This mechanical approach avoids the anisotropic effects and chemical alterations caused by thermal or chemical processes, maintaining material isotropy while achieving precise zone definition.

The analysis zones are pre-formed with precise geometry through mechanical punching before the actual analysis procedure. The punch tool creates zones with exact dimensions and shapes, eliminating the need for subsequent wax impregnation to define boundaries. This preliminary mechanical formation ensures both precise volume control and adequate lateral diffusion barriers through the matrix structure itself.

The patent eliminates the lamination process entirely by using direct mechanical punching to form analysis zones in the matrix. This substitution removes the tensile forces applied during lamination that cause anisotropic properties. The mechanical punching method achieves analysis zone formation without introducing directional stress or altering material isotropy.

The patent replaces solvent injection and evaporation processes with direct mechanical punching. This substitution eliminates the need for solvent application and subsequent evaporation, thereby removing the source of anisotropic properties caused by directional air flow. The mechanical punching method directly forms analysis zones with isotropic material properties.