Biochip Markers and Positioning Sequences for Spatial Matching
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Solution Overview
Problem
Existing biochips in spatial and spatiotemporal omics technologies face challenges in distinguishing and accurately matching different chips during manual or automated operations, leading to difficulties in establishing correspondence between captured products and detected biological tissues.
Innovation Solution
A biochip with a solid support featuring an array of feature sites and bio-molecular sequences, including positioning sequences and markers (human-eye and instrument-readable) to facilitate chip recognition and spatial position restoration, enabling accurate chip identification and biochemical reaction management.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If multiple biochips with similar appearances are used for spatial omics processing, then the biochemical reaction capacity increases, but the difficulty of distinguishing and matching different chips increases
Solution Approach 1:
The patent applies visual markers (color changes) on the biochip surface to enable easy distinction between different chips. The markers include colored patterns, codes, or visual indicators that allow operators to quickly identify and match chips with their corresponding data without confusion, directly resolving the chip distinction difficulty while maintaining multi-chip processing capacity
2Productivity
If manual or automated operations are performed on multiple similar-looking chips, then the processing throughput increases, but the matching accuracy between chips and data decreases
Solution Approach 1:
The patent implements a feedback mechanism where each biochip has unique identification markers that are read by automated systems to verify chip identity and establish correct matching with corresponding data. This feedback loop ensures matching accuracy is maintained even as processing throughput increases through automated operations
Solution Approach 2:
The patent uses visual copies (markers and codes) of chip identification information that can be rapidly read by automated systems. These visual copies enable high-speed automated matching operations without sacrificing accuracy, allowing throughput to increase while maintaining precision
3Ease of operation
If visual markers are added to biochips for identification, then the ease of operation improves, but the device complexity increases
Solution Approach 1:
The patent segments the biochip surface into functional regions: active areas for biochemical reactions and separate identification areas for markers. This segmentation allows visual markers to be added without interfering with the biochemical functionality, maintaining ease of operation while minimizing impact on device complexity
Data Source
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AI summary
Provided are a biochip (100) and a method of using and preparing a biochip. The biochip (100) includes: a solid support having a front surface (101) and a back surface (102); a plurality of feature sites (110) arranged in an array and a plurality of bio-molecular sequences attached to the plurality of feature sites (110) formed on the front surface, each bio-molecular sequence includes at least a positioning sequence, the positioning sequence includes information indicating a spatial position of the bio-molecular sequence on the biochip (100); and at least one of a first marker (120) recognizable by human eyes and a second marker (130) recognizable by an instrument provided on the back surface.