3D Bioprinted Biodressing Shape Maintenance via Calcium Chloride Timing
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Solution Overview
Problem
Existing methods for creating 3D biodressings face challenges in maintaining the structural integrity of biomaterials like sodium alginate while ensuring cell viability and therapeutic potential, especially when used for treating chronic wounds and severe burns.
Innovation Solution
A process involving the use of mesenchymal cells derived from the human umbilical cord or adipose tissue, cultured in xenoantigen-free human AB serum, combined with 4% sodium alginate, and produced through 3D bioprinting, where the calcium chloride solution is applied after a 5-15 minute pause post-bioprinting to enhance maturation and prevent deformation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If calcium chloride solution is applied immediately after bioprinting, then the structural integrity of sodium alginate is improved, but the 3D biodressing deforms and loses its shape
Solution Approach 1:
The patent applies preliminary action by allowing the bioprinted biodressing to rest for 5-15 minutes before applying calcium chloride solution. This pause enables the sodium alginate to initially set and maintain its printed shape, preventing deformation that would occur with immediate calcium chloride application. The preliminary resting period establishes a stable structure that can then be properly crosslinked.
2Stability of the object's composition
If mesenchymal cells are mixed with sodium alginate biomaterial, then the 3D biodressing structure is improved, but cell viability decreases
Solution Approach 1:
The patent uses sodium alginate as an intermediary material that facilitates the integration of mesenchymal cells into the 3D biodressing structure. The alginate forms a hydrogel matrix that provides structural stability while being biocompatible enough to maintain cell viability. This intermediary approach allows cells to be incorporated without direct harmful interactions, resolving the contradiction between structural integrity and cell survival.
3Productivity
If xenoantigen-containing culture medium is used, then cell proliferation is improved, but immunogenicity and safety are worsened
Solution Approach 1:
The patent applies parameter changes by transitioning from xenoantigen-containing culture media to xenoantigen-free culture media for mesenchymal cell cultivation. This parameter change eliminates the immunogenicity issue while maintaining cell proliferation and therapeutic potential. The modification of the culture medium composition allows the biodressing to be safer for clinical application without sacrificing cell productivity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The proposed process effectively maintains the shape of the 3D biodressing, ensures high cell viability for up to 15 days, and retains the therapeutic properties of mesenchymal cells, thereby enhancing wound healing and reducing treatment costs.
Implementation Method 1
coating the 3D biodressing with a 100 mM calcium chloride solution
Implementation Method 2
biomaterial, such as sodium alginate produced by the technique of three-dimensional bioprinting
Data Source
AI summary
The present invention refers to a process to obtain a three-dimensional (3D) biodressing comprising the steps of (a) isolating and culturing mesenchymal cells with human AB serum; (B) performing three-dimensional (3D) bioprinting using a biomaterial such as sodium alginate and cells obtained in step “a”; (c) putting at rest the 3D biodressing obtained after bioprinting; (d) covering the 3D biodressing with a 100 mM calcium chloride solution; (e) washing the 3D biodressing; (f) adding Dulbecco's Modified Eagle Medium (DMEM); and (g) keeping the biodressing in an incubator for up to 15 days. Additionally, the present invention refers to the 3D biodressing obtained comprising, preferably 4% sodium alginate and mesenchymal cells from the umbilical cord (MCUs), which has healing, anti-inflammatory, and analgesic properties. Furthermore, the present invention relates the 3D biodressing to treat patients with chronic wounds and severe burns.