Biofertilizer Consortium with Sodium Alginate for Cell Viability
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Solution Overview
Problem
Current biofertilizers have low cell density and short storage life, leading to decreased agricultural output despite their application, as they often degrade quickly due to low humidity in solid or powder forms, resulting in steep declines in viability and effectiveness.
Innovation Solution
A biofertilizer consortium comprising Pseudomonas stutzeri, Pseudomonas denitrificans, Pseudomonas resinovorans, Pseudomonas brassicacearum, Pseudomonas fluorescens, Shimwellia blattae, and Klebsiella oxytoca, formulated with a vehicle containing dehydrated milk whey, potassium chloride, and cell protectants like sodium alginate, maintaining high cell density and extending storage life to at least eight months.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If biofertilizer is formulated in solid or powder form, then it is easier to store and transport, but cell viability decreases rapidly due to low humidity
Solution Approach 1:
The patent changes the physical state parameter of the biofertilizer from solid/powder to liquid form, which maintains higher humidity levels necessary for preserving bacterial cell viability during storage and transport
Solution Approach 2:
The patent creates a composite liquid formulation combining multiple bacterial strains (Pseudomonas stutzeri, P. denitrificans, P. resinovorans, P. brassicacearum, P. fluorescens, Shimwellia blattae, and Klebsiella oxytoca) with protective carriers and nutrients, resulting in a stable liquid biofertilizer that maintains both ease of application and high cell viability
2Ease of operation
If conventional biofertilizer formulations are used, then application is simple, but storage life is short (less than eight months) due to rapid degradation
Solution Approach 1:
The patent incorporates cell protectants and protective carriers in the liquid formulation before storage, which cushion and protect the bacterial cells from degradation during storage, extending shelf life to at least eight months while maintaining simplicity of application
Solution Approach 2:
The patent uses protective carriers as intermediaries between the bacterial cells and the storage environment, shielding the cells from harmful conditions during storage while allowing easy application to crops
3Duration of action of stationary object
If cell density is reduced to extend storage life, then storage stability improves, but agricultural output decreases
Solution Approach 1:
The patent changes the formulation parameters to create a liquid biofertilizer that maintains high cell density (1×10^9 CFU/mL) while achieving extended storage stability of at least eight months, thereby preserving both productivity and storage properties
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The biofertilizer consortium maintains high cell viability and density, significantly increasing crop yields by up to 59% and extending shelf life, demonstrating improved agricultural output and storage stability.
Implementation Method 1
cell protectants like sodium alginate
Implementation Method 2
formulated with a vehicle containing dehydrated milk whey
Data Source
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AI summary
This invention consists of a consortium of plant-growth promoting microorganisms including Pseudomonas stutzeri, Pseudomonas denitrificans, Pseudomonas resinovorans, Pseudomonas brassicacearum, Pseudomonas fluorescens, Shimwellia blattae and Klebsiella oxytoca. The bacterial consortium along with a vehicle suitable for agricultural application, form a biofertilizer useful to enhance agricultural yield when applied to cultivation plants. The biofertilizer described present a long shelf life and preserve a high cell density along the time.