Optimizing Sulfur Autotrophic Denitrifying Bacteria Biofilm Preservation Temperature
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Solution Overview
Problem
Current methods for determining the optimal preservation temperature of sulfur autotrophic denitrifying bacteria biofilms are lacking, which hinders the efficient recovery of microbial activity and application in sewage treatment plants, leading to increased operational costs and extended startup times.
Innovation Solution
A method using flow cytometry to characterize the cell activity state of sulfur autotrophic denitrifying bacteria biofilms at different temperatures, identifying the optimal preservation temperature by determining the proportions of living, early apoptotic, late apoptotic, and dead cells, and applying this information to streamline the microbial activity recovery process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If sulfur autotrophic denitrifying bacteria biofilm is preserved without determining optimal temperature, then preservation can be performed, but microbial activity recovery time is extended and operational costs increase
Solution Approach 1:
The patent applies preliminary action by determining the optimal preservation temperature (-20°C) through preliminary experiments using flow cytometry to characterize cell activity states. This preliminary determination of optimal conditions before actual preservation use allows the biofilm to be stored effectively, reducing recovery time when deployed without requiring complex real-time monitoring during storage.
2Measurement precision
If flow cytometry is used to characterize cell activity state, then optimal preservation temperature can be determined, but measurement complexity and equipment requirements increase
Solution Approach 1:
The patent replaces complex mechanical monitoring systems with flow cytometry, which uses optical detection (laser excitation and fluorescence detection) to characterize cell activity states. This substitution provides precise measurement of cell viability and activity through fluorescent markers while avoiding the need for complex mechanical sensors and actuators that would be required for alternative measurement approaches.
3Productivity
If optimal preservation temperature is determined, then startup time of engineering application is shortened, but research and development investment increases
Solution Approach 1:
The patent applies parameter changes by systematically varying preservation temperature as the key parameter and using flow cytometry to measure its effect on cell activity state. By identifying -20°C as the optimal temperature parameter, the patent enables faster startup of engineering applications. The research investment is concentrated in the initial parameter optimization phase, after which the knowledge can be applied repeatedly without additional cost.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for rapid startup of sulfur autotrophic denitrifying bacteria biofilm projects, reduces operational costs, and achieves energy-saving and consumption-reducing effects by determining the optimal preservation temperature, thereby enhancing the efficiency of nitrate and total nitrogen removal in sewage treatment plants.
Implementation Method 1
determining the cell activity state of a sulfur autotrophic denitrifying bacteria biofilm preserved at different temperatures by flow cytometry
Data Source
AI summary
The present disclosure discloses to a method for determining optimum preservation temperature of a sulfur autotrophic denitrifying bacteria biofilm, and belongs to the technical field of environment engineering. The method of the present disclosure comprises: determining the cell activity state of a sulfur autotrophic denitrifying bacteria biofilm preserved at different temperatures by flow cytometry, and determining the preservation temperature of the cell activity state closest to the cell activity state of the sulfur autotrophic denitrifying bacteria in pilot operation as the optimum preservation temperature. The cell activity state and performance effect are verified to be reliable after activity recovery by the test data. The method of the present disclosure can simplify the microbial activity recovery process of the sulfur autotrophic denitrifying bacteria biofilm, quickly start the sulfur autotrophic denitrifying bacteria biofilm sewage treatment, enable the removal rate of nitrate nitrogen and total nitrogen in a sewage treatment plant to reach 96% and 88% or above respectively, achieve the effects of energy saving and consumption reduction at the same time, and have very high industrial feasibility.