Bioink for 3D Bioprinted Bone Models with Channels
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Solution Overview
Problem
Current in vitro bone models fail to accurately mimic the native bone environment, limiting their effectiveness in studying bone-related diseases and tissue engineering applications.
Innovation Solution
Development of a bioink that includes a plurality of bone-related cells, such as osteoblasts, osteoclasts, and bone marrow cells, combined with materials like hydroxyapatite and fibrin, to create a three-dimensional artificial bone structure with channels for fluid flow, mimicking the bone's native environment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional two-dimensional cell culture models are used, then the model simplicity and ease of operation are maintained, but the ability to mimic native bone environment and provide realistic physiological conditions deteriorates
Solution Approach 1:
The patent transitions from traditional two-dimensional cell culture to three-dimensional bioprinted bone models. The bioink formulation enables cells to be embedded in a three-dimensional matrix that mimics the native bone extracellular matrix, providing realistic physiological conditions while maintaining operational feasibility through automated bioprinting systems
Solution Approach 2:
The patent develops a composite bioink formulation combining hydrogels (such as alginate, gelatin, or collagen) with bone-related materials (such as hydroxyapatite particles or bone-derived extracellular matrix). This composite structure provides both the three-dimensional architecture needed for realistic bone environment mimicry and the mechanical properties required for model stability
2Reliability
If three-dimensional bioprinted bone models with multiple cell types and bioink materials are created, then the realism and physiological relevance of the bone model is improved, but the manufacturing complexity and process difficulty increase
Solution Approach 1:
The patent performs preliminary formulation and optimization of the bioink composition before the actual bioprinting process. The bioink is pre-mixed with appropriate concentrations of hydrogels, bone materials, and growth factors, and pre-tested for printability and cell viability. This preliminary preparation simplifies the manufacturing process by eliminating the need for complex real-time adjustments during bioprinting
Solution Approach 2:
The patent optimizes key parameters of the bioink formulation, including viscosity, crosslinking density, and material composition ratios, to achieve the desired balance between printability and physiological realism. By systematically adjusting these parameters, the patent creates a bioink that is both easy to print with and highly effective at mimicking native bone environment
3Reliability
If conventional bone models without specialized bioink formulations are used, then the manufacturing process is simpler, but the cell viability and proliferation capacity deteriorate
Solution Approach 1:
The patent introduces a specially formulated bioink as an intermediary material between the cells and the bioprinting process. This bioink contains bioactive components such as growth factors, cytokines, or extracellular matrix proteins that mediate cell survival, proliferation, and differentiation signals. The bioink acts as a protective and supportive environment for cells during and after the bioprinting process
Solution Approach 2:
The patent modifies the chemical and physical parameters of the bioink formulation to enhance cell viability and proliferation. This includes adjusting the hydrogel composition to provide appropriate mechanical support, incorporating bone-derived materials to provide cell-adhesive ligands, and optimizing the porosity and degradation rate to match native bone tissue characteristics
Data Source
AI summary
Embodiments pertain to a bioink operational to form an artificial bone. The bioink includes a plurality of bone-related cells. Additional embodiments pertain to an artificial bone that includes a plurality of bone-related cells; and a bioink of the present disclosure that is embedded with the cells. The artificial bone may be in the form of a three-dimensional structure that includes a plurality of channels operational to allow fluid flow through the artificial bone. Further embodiments pertain to methods of making an artificial bone by applying a plurality of bone-related cells and a bioink onto a surface such that the bioink becomes embedded with the cells and forms a three-dimensional structure with a plurality of channels.