Biological Disgregation Device Using Perforated Grid
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional biological material disgregation methods alter the form, function, and viability of cells, requiring the use of chemical reagents to prepare samples and tissue micrografts for analysis and therapy, leading to uncertain data and modified biological samples.
Innovation Solution
A disgregating device with a perforated grid featuring microholes of optimized size and configuration, manufactured through a specialized process, that maintains cell viability and integrity by allowing cells to pass through without chemical reagents, enabling the preparation of cell suspensions and tissue micrografts for various applications.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional disgregation methods are used to prepare biological samples, then the disgregation process can be completed, but the cells undergo alterations in form, function, and viability requiring chemical reagents
Solution Approach 1:
The patent replaces conventional mechanical disgregation methods (which cause cell damage) with a chemical-free disgregation system using a specifically designed grid with microholes. The grid structure enables mechanical separation without the need for chemical reagents, thereby maintaining cell viability while achieving effective disgregation. This substitution of mechanical action with a structured physical barrier resolves the contradiction between reliable cell preservation and ease of sample preparation.
2Measurement precision
If chemical reagents are used to prepare cell suspensions, then sample preparation can be achieved, but data accuracy and biological sample integrity become uncertain
Solution Approach 1:
The patent employs a grid with specifically designed microholes (porous structure) to achieve chemical-free disgregation. The porous grid allows cells to pass through while maintaining their integrity, eliminating the need for chemical reagents that would compromise data accuracy. The porous material approach resolves the contradiction by providing a physical separation mechanism that ensures measurement precision while managing device complexity through a relatively simple grid structure.
3Productivity
If conventional disgregation devices are used, then biological material can be processed, but cell form and viability are altered
Solution Approach 1:
The patent applies local quality by designing a grid with microholes of specific dimensions and configurations tailored to different cell types and disgregation requirements. Rather than using a uniform aggressive mechanical approach, the local quality of the grid structure (hole size, shape, and distribution) enables gentle yet effective disgregation that maintains cell structure integrity while achieving productivity. This localized optimization resolves the contradiction between disgregation efficiency and cell structure stability.
Data Source
AI summary
A disgregating device of biological material, comprising: a hollow outer body, defining an inner chamber; a fixed disgregating grid, having a plurality of microholes provided with sharp edges, and housed transversely in the inner chamber so as to define an upper loading chamber apt to be loaded with the biological material to be disgregated and a lower collecting chamber apt to collect the biological material, after it has been disgregated; and a bladed rotor, rotating in the inner chamber, apt to co-operate, rotating, with the fixed disgregating grid, so as to feed and bring the biological material, contained in the upper loading chamber, into contact with the microholes of the disgregating grid and therefore cause the disgregation of the biological material making it pass through these microholes.