Biological Nitrogen Fixation Bioreactor for Sustainable Fertilizer Production

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Solution Overview

Problem

Current methods for producing mineral nitrogen for non-legume plants are energy-intensive and costly, relying on chemical processes that require high temperatures and pressures, making them unsustainable and expensive for farmers.

Innovation Solution

A biological process using nitrogen-fixing bacteria and bioreactors to convert atmospheric dinitrogen into assimilable mineral nitrogen, including ammonia and nitrates, through a series of incubation steps with organic matter and specific bacterial genera, such as Azotobacter and Nitrosomonas, which also produces dihydrogen as a byproduct.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If chemical processes are used to produce ammonia from nitrogen and hydrogen, then nitrogen fertilizer can be produced, but energy consumption is very high and equipment operating costs are high

Engineering Contradiction:
Improveammonia productionVSAvoidenergy consumption
Core Design Contradiction:
Quantity of substanceVSUse of energy by moving object

Solution Approach 1:

The patent replaces the mechanical/chemical Haber-Bosch process with a biological system using nitrogen-fixing bacteria that naturally convert atmospheric nitrogen into ammonia through enzymatic reactions, eliminating the need for high-pressure compressors, heaters, and complex chemical reaction vessels

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The nitrogen-fixing bacteria perform the nitrogen fixation function autonomously using organic matter as carbon source and energy, converting atmospheric nitrogen into ammonia without external energy input for heating or pressurization, making the system self-sufficient

Inventive Principle:
Principle #25Self-service

2Quantity of substance

If chemical processes are used to produce nitrogen fertilizers, then assimilable nitrogen can be produced, but production costs are very significant

Engineering Contradiction:
Improvemineral nitrogen productionVSAvoidproduction cost
Core Design Contradiction:
Quantity of substanceVSEase of manufacture

Solution Approach 1:

The patent uses inexpensive organic matter (such as agricultural waste, food waste, or simple carbon sources) as the energy and carbon source for nitrogen-fixing bacteria, replacing expensive fossil fuels and specialized chemicals required in conventional processes

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The patent introduces nitrogen-fixing bacteria as a biological intermediary that mediates the conversion of atmospheric nitrogen into ammonia, replacing direct chemical reactions and enabling the process to occur under mild, cost-effective conditions

Inventive Principle:
Principle #24Intermediary (Mediator)

3Quantity of substance

If chemical processes are used to produce ammonia, then nitrogen fertilizer is obtained, but the process requires high temperature and high pressure equipment

Engineering Contradiction:
Improveammonia productionVSAvoidequipment complexity
Core Design Contradiction:
Quantity of substanceVSDevice complexity

Solution Approach 1:

The patent replaces complex mechanical equipment (high-pressure compressors, high-temperature reactors, heat exchangers, safety valves) with simple biological vessels containing nitrogen-fixing bacteria that operate at ambient temperature and pressure

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the operating parameters from extreme conditions (200-400 bars, 450°C) to mild biological conditions (ambient temperature and pressure), fundamentally simplifying the equipment requirements for ammonia production

Inventive Principle:
Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This method reduces energy consumption and production costs, allowing farmers to produce nitrogenous fertilizers on-site, while also generating hydrogen for energy use, providing a sustainable and cost-effective alternative to traditional chemical processes.

Implementation Method 1

Incubating organic matter, nitrogen (N2), a simple sugar source, and nitrogen-fixing bacteria in a bioreactor to obtain a fermentation medium containing ammonia (NH3)

Methodology Applied
Scientific EffectNitrogen fixation: Fermentation

Implementation Method 2

Incubating the fermentation medium obtained in step a) in the presence of water and with removal of hydrogen (H2) from the bioreactor to obtain a fermentation medium containing ammonium (NH4+)

Methodology Applied
Scientific EffectDissolution and chemical reaction: Solvation

Implementation Method 3

Incubating the fermentation medium obtained in step b) in the presence of oxygen (O2) and bacteria of the genus Nitrosomonas and/or Nitrobacter in the bioreactor to obtain a fermentation medium containing nitrates (NO3-)

Methodology Applied
Scientific EffectNitrification: Oxidation

Data Source

PatentEP3620507A1Biological method for producing mineral nitrogen and/or dihydrogen from atmospheric dinitrogen
Publication Date: 2020.03.11 MEC
  • EP3620507A1 patent drawingFigure 1
  • EP3620507A1 patent drawingFigure 2
  • EP3620507A1 patent drawingFigure 3

AI summary

The present invention relates to a biological process for producing mineral nitrogen and/or dihydrogen from atmospheric dinitrogen, using successively nitrogen-fixing bacteria and bacteria of the genus Nitrosomonas and/or Nitrobacter. The present invention also relates to a suitable device for implementing this process, comprising at least three successive compartments. The present invention particularly relates to a device comprising a bioreactor (1), said bioreactor comprising a fermentation vessel (24) having at least three successive compartments (3, 4, 5). The present invention also relates to the use of nitrogen-fixing bacteria and/or bacteria of the genus Nitrosomonas and/or Nitrobacter for the production of mineral nitrogen and/or dihydrogen.