Biological Particle Detection via Integrated Optical Screening and Capture
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Solution Overview
Problem
Conventional biochemical detecting methods are limited by operator skills and tools, requiring large specimen samples and multiple apparatuses, making microdetection inefficient and prone to sample deterioration and contamination, with no method to locate and capture single specific biological particles without pre-processing.
Innovation Solution
A system comprising a dilution apparatus with a microfluidic channel and a selectively capturing apparatus, using optical detection modules to quickly and precisely detect and capture target biological particles within a sample, avoiding the need for sample transfer and subsequent contamination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional biochemical detecting method is used with manual operation and multiple apparatuses, then detection accuracy can be improved through sequential screening and high-accuracy detection, but sample deterioration and contamination occur during transfer, and detection time increases to several minutes to hours
Solution Approach 1:
The patent merges the screening function and high-accuracy detection function into a single integrated apparatus. The imaging device captures images of biological particles, and the processor simultaneously performs both screening (identifying candidate particles) and high-accuracy detection (precise measurement of target particles) without requiring physical transfer of samples between different devices, thereby preventing sample deterioration and contamination while maintaining detection accuracy.
Solution Approach 2:
The apparatus is designed with multi-functionality, where the same imaging device and sample chamber serve dual purposes: initial screening of biological particles and subsequent high-accuracy detection of target particles. The processor distinguishes between screening mode and detection mode, allowing one apparatus to perform multiple functions that previously required separate devices, thus eliminating transfer-related contamination and deterioration.
2Measurement precision
If manual operation and sample transfer between apparatuses are performed, then detection process can be completed with conventional methods, but detection time increases to several minutes to hours and sample is probably deteriorated and contaminated
Solution Approach 1:
The patent combines screening and high-accuracy detection into a single integrated operation within one apparatus. The imaging device continuously monitors the sample, and the processor identifies target particles during the same time frame and location where screening occurs, eliminating the time-consuming transfer process between separate apparatuses while maintaining both screening efficiency and detection accuracy.
Solution Approach 2:
The system enables continuous detection without interruption or transfer. The imaging device maintains continuous observation of the sample in the sample chamber, and the processor continuously analyzes images to identify and measure target particles. This continuous action eliminates the breaks and transfers that previously caused time loss and sample deterioration, achieving both speed and accuracy.
3Ease of operation
If great amount of specimen is required for conventional biochemical detecting method, then detection can be performed with manual operation, but microdetection is hard to be done and sample consumption increases
Solution Approach 1:
The patent replaces manual mechanical operations with an automated imaging and processing system. The imaging device automatically captures images of biological particles, and the processor automatically identifies, screens, and measures target particles based on image analysis. This substitution of manual mechanical detection with optical imaging and computational processing enables precise detection of micro-quantities of samples that would be impossible to detect manually, significantly reducing sample consumption while maintaining ease of operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient detection and capture of single target biological particles from large samples without deterioration, enhancing detection efficiency and reliability by maintaining the sample in a stable environment throughout the process.
Implementation Method 1
The first optical detection module provides a first optical pathway. The first optical pathway passes through the microfluidic channel and is adapted to detect the target biological particles.
Implementation Method 2
The second optical detection module locates the accurate position of each of the target biological particles on the sample tray.
Data Source
AI summary
A system for detecting biological particles includes a dilution apparatus and a selectively capturing apparatus. The dilution apparatus includes a microfluidic channel, a sample tray assembly, and a first optical detection module. The microfluidic channel is connected to a sample reservoir adapted to supply sample containing the target biological particles. The sample tray assembly includes a sample tray for containing sample discharged through the microfluidic channel. The first optical detection module provides a first optical pathway passing through the microfluidic channel. When the target biological particles that flow through the microfluidic channel are detected by the first optical detection module, the sample tray assembly provides the sample tray for loading the target biological particles that flow through the microfluidic channel. The sample tray containing the target biological particles is moved to the selectively capturing apparatus. After a second optical detection module locates an accurate position of each of the target biological particles on the sample tray, the capturing device is moved to the accurate position of each of the target biological particles to capture the target biological particles into a collection plate.