Biological Sample Analysis Device Threshold-Based Reference Remeasurement
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Solution Overview
Problem
The mobility of DNA fragments in electrophoresis is significantly affected by temperature, pressure, pH, reagent deterioration, and other environmental factors, leading to variations in analysis results and the need for unnecessary reacquisition of reference data, which increases costs and reduces system throughput.
Innovation Solution
A biological sample analysis device that compares first measurement data from a biological sample with second measurement data from a reference sample, determining if the difference exceeds a threshold value, thereby deciding whether to remeasure the reference sample before analyzing the biological sample.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If reference data is reacquired frequently to ensure analysis accuracy, then measurement precision is improved, but productivity deteriorates due to increased remeasurement time
Solution Approach 1:
The system implements feedback by comparing measurement data from biological samples with reference data, calculating differences, and using this feedback to determine whether remeasurement of reference samples is necessary. This feedback mechanism enables dynamic adjustment of remeasurement frequency based on actual data quality, resolving the contradiction between maintaining high analysis accuracy and preserving system throughput.
Solution Approach 2:
The invention introduces dynamic decision-making for remeasurement based on calculated differences between measurement data and reference data. Instead of fixed-frequency remeasurement, the system adaptively determines remeasurement necessity by comparing actual data variations against thresholds, thereby optimizing the balance between accuracy maintenance and productivity preservation.
2Reliability
If reference data is reacquired frequently to maintain accuracy, then reliability is improved, but loss of time increases due to repeated remeasurement
Solution Approach 1:
The system uses feedback from data comparison to intelligently determine remeasurement timing. By continuously monitoring differences between measurement data and reference data, the system only triggers remeasurement when actual accuracy degradation occurs, rather than following a fixed schedule. This reduces unnecessary time loss while maintaining reliability.
Solution Approach 2:
The system performs self-diagnosis by automatically comparing its own measurement data against reference data and determining whether remeasurement is needed. This self-service capability eliminates the need for external intervention or fixed-schedule remeasurement, reducing time loss while maintaining analysis reliability through on-demand reference data validation.
3Measurement precision
If environmental conditions are strictly controlled to maintain mobility consistency, then measurement precision is improved, but device complexity increases
Solution Approach 1:
Instead of relying solely on complex environmental control systems, the invention introduces feedback through data comparison between measurements and reference data. This feedback mechanism detects actual mobility variations caused by environmental fluctuations and triggers reference data remeasurement when necessary, providing a simpler software-based solution to maintain precision without over-engineering environmental controls.
Solution Approach 2:
The invention introduces an intermediary data comparison mechanism that mediates between environmental variations and measurement accuracy. Rather than directly controlling all environmental parameters, the system uses reference data as an intermediary standard to detect and compensate for environmental effects, simplifying the overall system while maintaining measurement precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach improves the accuracy of analysis results, reduces reagent costs, and increases system throughput by minimizing unnecessary remeasurement of reference samples.
Implementation Method 1
Electrophoresis is performed to measure the fragment length of the target DNA fragment obtained by PCR. The electrophoresis is a DNA fragment separation method utilizing the fact that a migration speed in a charged migration path differs depending on the length of the DNA fragment
Implementation Method 2
Each sample, that is, each DNA fragment is labeled with a fluorescent dye, and a fluorescent signal of the migrated sample is detected by an optical detector placed at the end of the capillary
Data Source
AI summary
The present invention aims to provide a biological sample analysis device which achieves an improvement in accuracy of an analysis result, a reduction in reagent cost, and shortening of a required time. The biological sample analysis device according to the present invention compares first measurement data acquired by measuring a biological sample and second measurement data acquired by measuring a reference sample and determines that when the difference between the two exceeds a threshold value, it is necessary to remeasure the reference sample before remeasuring the biological sample and reacquire a reference value (refer to FIG. 3A).