Biological Sample Analysis System Time Gate Setting
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Solution Overview
Problem
Existing biological sample analysis systems face challenges in appropriately setting the timing for acquiring data related to light generated from biological particles, which is crucial for accurate analysis and sorting.
Innovation Solution
A biological sample analysis system that includes a light irradiation unit, a detection unit, and an information processing unit. The information processing unit sets a section defining the time for acquiring data related to light generated by light irradiation at one or more irradiation points different from a reference point, based on changes in light data.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the time gate for data acquisition is set too broadly, then more light data is acquired, but the statistical accuracy of particle analysis deteriorates
Solution Approach 1:
The patent applies preliminary action by calculating flight time from excitation light detection to sorting light detection before the actual particle analysis. This pre-calculated timing information is used to set the time gate window, ensuring that data acquisition is precisely timed to capture only the relevant light signals from particles at the sorting position, thereby improving statistical accuracy without acquiring excessive data
Solution Approach 2:
The patent changes the parameter of time gate positioning based on calculated flight time. By dynamically adjusting the time gate window according to the specific flight time of particles (determined by flow velocity and distance), the system optimizes the data acquisition timing to capture the maximum relevant signal while minimizing noise and irrelevant data, thus improving measurement precision
2Adaptability or versatility
If the time gate is set based on fixed timing, then the system is simple to operate, but it cannot adapt to variations in particle flow velocity
Solution Approach 1:
The patent implements feedback by using the detected sorting light (which confirms particle arrival at the sorting position) to refine and update the time gate settings. The system continuously adjusts the time gate window based on actual particle arrival times feedback from the detection unit, enabling adaptation to flow velocity variations while maintaining a relatively simple control architecture
Solution Approach 2:
The system performs preliminary calculation of flight time using the known distance between irradiation points and measured particle velocity from the time difference between excitation light and sorting light detection. This pre-established timing model provides a foundation for adaptive time gate setting without requiring complex real-time control mechanisms
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for efficient and appropriate setting of the time gate for data acquisition, reducing the amount of data required and improving the statistical accuracy of biological particle analysis and sorting.
Implementation Method 1
a light irradiation unit that irradiates each particle flowing through a flow channel with light
Implementation Method 2
a particle population such as cells, microorganisms, and liposomes is labeled with a fluorescent dye, and the intensity and/or pattern of fluorescence generated from the fluorescent dye excited by irradiating each particle of the particle population with laser light is measured
Data Source
AI summary
An object of the present disclosure is to provide a technology for appropriately setting a timing of acquiring data related to light generated from biological particles.The present disclosure provides a biological sample analysis system including: a light irradiation unit designed to irradiate each particle flowing through a flow channel with light at a plurality of irradiation points; a detection unit that detects light generated when each of the particles passes through each of the plurality of irradiation points; and an information processing unit that processes data related to light detected by the detection unit, in which the information processing unit is designed to perform processing of setting a section that defines a time for acquiring data related to light generated by light irradiation at an irradiation point different from a reference irradiation point, and the information processing unit executes the section setting processing on the basis of a change in data related to light accompanying a change in the section.