Biological sample cooling device and multifunctional cooling system
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Solution Overview
Problem
Existing biological sample storage methods fail to achieve slow gradient cooling, leading to potential damage from rapid temperature changes, particularly when using liquid nitrogen.
Innovation Solution
A biological sample cooling device with a cooling adjusting assembly and circulation cooling assembly that adjusts temperature gradients by heating gas to mix with liquid nitrogen, ensuring slow cooling and temperature control for multiple samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If liquid nitrogen is used directly for cooling biological samples, then cooling efficiency is improved, but sample damage occurs due to rapid temperature changes
Solution Approach 1:
The patent introduces a gas-phase nitrogen intermediary between liquid nitrogen and biological samples. The system vaporizes liquid nitrogen to create a gas-phase cooling medium that indirectly cools samples, preventing direct thermal shock while maintaining cooling effectiveness through controlled heat transfer
Solution Approach 2:
The system dynamically adjusts cooling parameters by controlling the phase transition of nitrogen (liquid to gas) and regulating gas flow rates. This allows precise control of temperature gradients, enabling slow gradient cooling that protects sample integrity while achieving desired cooling speeds
2Quantity of substance
If multiple biological samples are stored together, then storage efficiency is improved, but temperature control precision deteriorates
Solution Approach 1:
The storage system is divided into multiple independent temperature zones, each capable of separate temperature control. Samples are distributed across different grillages and zones, allowing individualized temperature management that maintains precision even when storing large quantities of samples simultaneously
Solution Approach 2:
The system employs a multi-functional temperature control mechanism that can simultaneously manage multiple zones with different temperature requirements. The circulation cooling assembly can selectively cool different regions, providing universal temperature control capability across the entire storage system
3Object-affected harmful factors
If liquid nitrogen temperature is increased to prevent sample damage, then sample safety is improved, but cooling capability deteriorates
Solution Approach 1:
The system implements periodic cycles of direct liquid nitrogen cooling and gas-phase cooling. During gas-phase phases, temperature is allowed to rise slightly to prevent sample damage; during liquid nitrogen phases, stronger cooling is applied. This periodic alternation maintains sample safety while preserving overall cooling capability
Solution Approach 2:
The circulation cooling assembly maintains continuous cooling action through the nitrogen vapor circulation system. Even when liquid nitrogen is not directly applied, the gas-phase nitrogen continues to absorb heat from samples, ensuring uninterrupted cooling protection that prevents sample inactivation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables intelligent temperature adjustment and slow gradient cooling, preventing sample inactivation and allowing multiple samples to be stored efficiently with controlled temperature adjustments.
Implementation Method 1
a gas in the cavity assembly is sucked into the heating module to be heated
Implementation Method 2
the cooling barrel member controls the temperature of the liquid nitrogen
Data Source
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AI summary
The present invention discloses a biological sample cooling device and a multi-functional cooling system. The biological sample cooling device comprises a cavity assembly, a cooling adjusting assembly, a circulation cooling assembly and biological grillages. The multi-functional cooling system comprises a transport assembly, a lifting assembly, a support rack assembly, a cover opening assembly, a rotatory liquid adding assembly, a grillage grabbing assembly, a pipette assembly, a sample temporary storage box, a barcode scanner and a transfer tank. The present invention has the beneficial effects that a whole-plate biological sample is slowly cooled through the cooling adjusting assembly; the temperature of liquid nitrogen is detected by the circulation cooling assembly; when the temperature is too low, a gas in the cavity assembly is sucked into a heating module to be heated, and multiple groups of biological samples are stored in the circulation cooling assembly; the lifting assembly drives the transfer tank to rise to be docked with the cavity assembly; and the biological grillages and biological single-tube samples are grabbed by utilizing the grillage grabbing assembly and the pipette assembly, thereby placing the biological grillages or the biological single-tube samples into the cooling adjusting assembly or the circulation cooling assembly, so as to achieve the cooling of the biological samples.