Biological Sample Stabilizer Composition for Room Temperature Storage
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Solution Overview
Problem
Biological samples, such as nucleic acids and polypeptides, are challenging to preserve over extended periods due to their labile nature, especially when storage requires subzero temperatures, which can be difficult to maintain, especially during transportation, and existing stabilization methods like lyophilization result in activity loss upon reconstitution.
Innovation Solution
A composition comprising a compound of Formula I, a precipitant, a lower alcohol, and a chaotrope, along with optional agents like a reducing agent, polymerase inhibitor, and pH buffer, is used to stabilize biological samples under non-freezing conditions, allowing for storage at room temperature without dehydration or extensive refrigeration.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If subzero temperature storage is used, then biological sample stability is improved, but storage cost and device complexity increase due to requiring refrigerating equipment
Solution Approach 1:
The invention changes the storage temperature parameter from subzero to room temperature by introducing a stabilizer composition containing compound of Formula I, precipitant, lower alcohol, and chaotrope. This chemical parameter change eliminates the need for refrigerating equipment while maintaining sample stability.
Solution Approach 2:
The stabilizer composition acts as an intermediary substance that mediates between the biological sample and the storage environment. It protects the sample from degradation at room temperature without requiring external refrigeration equipment.
2Duration of action of stationary object
If lyophilization is used, then storage duration is improved, but biological activity is lost upon reconstitution
Solution Approach 1:
The invention changes the physical state parameter from dried (lyophilized) to liquid form by using a stabilizer composition that maintains samples in liquid state at room temperature. This eliminates the need for reconstitution while extending storage duration and preserving biological activity.
3Device complexity
If room temperature storage without stabilizer is used, then device complexity is reduced, but sample degradation increases
Solution Approach 1:
The stabilizer composition serves as a chemical intermediary that protects the biological sample from degradation at room temperature. It includes compound of Formula I, precipitant, lower alcohol, and chaotrope that work together to maintain sample integrity without requiring refrigeration equipment.
4Reliability
If international transportation with refrigeration is used, then sample stability is improved, but energy consumption and cost increase
Solution Approach 1:
The invention changes the storage temperature parameter from subzero to room temperature, eliminating the need for energy-consuming refrigeration equipment during international transportation while maintaining sample stability through the stabilizer composition.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composition effectively maintains the integrity and stability of biological samples for extended periods, reducing degradation and preserving activity, making it suitable for long-term storage and transportation without the need for expensive refrigeration equipment.
Implementation Method 1
a composition for storage of a biological sample, particularly a composition for storage of a nucleic acid and/or a polypeptide in a biological sample under non-freezing conditions
Implementation Method 2
the composition comprises: 1) at least one compound of Formula I; 2) a precipitant
Implementation Method 3
the composition comprises: 1) at least one compound of Formula I; 2) a precipitant; 3) a lower alcohol; and 4) a chaotrope
Data Source
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AI summary
Disclosed are a stabilizer for storage of a biological sample, a composition for storage of a nucleic acid and/or a polypeptide in a biological sample under non-freezing conditions, a kit comprising the composition, a mixture comprising the composition and a biological sample, and a method for storage of a nucleic acid and/or a polypeptide in a biological sample under non-freezing conditions. The integrity and stability of the nucleic acid and/or polypeptide in a biological sample can be stored for a long time under non-freezing conditions by using the stabilizer according to the invention, and therefore the stabilizer has a good application prospect.