Biological Valve Calcification Evaluation Method
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Solution Overview
Problem
Current methods for evaluating biological valve calcification are inefficient, costly, and technically challenging, requiring animal clinical experiments that are time-consuming and prone to variability, making it difficult to accurately assess the anti-calcification properties of biological valves.
Innovation Solution
An in-vitro biological valve calcification evaluation method using a calcification evaluation solution comprising glutaraldehyde, saline, borate buffer, reducing calcium ingredient solution, and calcium-containing plasma, which involves preheating, chemical treatment, and an in-vitro calcification reaction to measure calcium content using atomic absorption spectroscopy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If animal clinical experiments are used to evaluate biological valve calcification, then the evaluation can reflect actual physiological conditions, but the evaluation period becomes extremely long (6 months to 1 year) and costs increase significantly
Solution Approach 1:
The patent creates an in-vitro calcification evaluation system that copies the essential chemical interaction between biological valve tissue and calcium ions without requiring whole animal systems. The evaluation solution contains plasma calcium ions, buffer solutions, and enzyme systems that replicate the calcification process in a controlled test tube environment, eliminating the need for long-term animal feeding experiments while preserving the core chemical evaluation mechanism
Solution Approach 2:
The patent extracts the essential calcification evaluation function from the complex animal clinical experiment system. By isolating the key chemical components (calcium ions, biological valve tissue, buffer system) and placing them in a simplified in-vitro environment, the patent removes the time-consuming physiological processes (feeding, growth, metabolism) while retaining the core calcification chemistry that needs to be evaluated
2Reliability
If animal clinical experiments are used to evaluate biological valve calcification, then comprehensive physiological effects can be observed, but technical difficulty and operational complexity increase greatly
Solution Approach 1:
The patent segments the evaluation system into distinct functional components: evaluation solution (containing calcium ions and buffer), biological valve sample, and detection system. This segmentation eliminates the need for complex animal surgery, implantation, and long-term monitoring procedures, reducing technical dependence on clinical surgical skills while maintaining evaluation reliability through controlled chemical interactions
Solution Approach 2:
The patent introduces an intermediary evaluation solution that mediates between the biological valve tissue and calcium ions. This solution contains buffer systems, plasma components, and enzyme preparations that facilitate the calcification process in a controlled manner, replacing the need for complex animal physiological systems while maintaining the essential chemical evaluation pathway
3Duration of action of stationary object
If long feeding periods are used in animal experiments, then complete calcification processes can occur, but variability from individual differences and environmental factors increases
Solution Approach 1:
The patent changes the evaluation parameters from long-term physiological observation to short-term controlled chemical reaction. By adjusting the evaluation solution composition (calcium ion concentration, buffer pH, enzyme activity) and incubation conditions (temperature, time), the patent achieves complete calcification process evaluation in hours rather than months, eliminating variability from individual animal differences and environmental factors while maintaining process completion
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method significantly shortens the evaluation period, reduces costs, and enhances the anti-calcification capacity of biological valves by chemically blocking calcium ion binding, providing a standardized and effective assessment of calcification performance.
Implementation Method 1
chemical treatment of the biological valve thereby blocking certain free chemical groups
Implementation Method 2
the calcium content in biological valve tissue is detected by atom absorption spectroscopy
Data Source
AI summary
An in-vitro biological valve calcification evaluation method, comprising the following steps: preparing calcification evaluation solution comprising glutaraldehyde solution, saline solution, borate buffer solution, reducing calcium ingredient solution and calcium-containing plasma solution, the reducing calcium ingredient solution comprising alcohol, alkaline solution, oil and its derivatives, and buffer solution; preheating the reducing calcium ingredient solution; conducting treatment with the glutaraldehyde solution prior to reducing calcium ingredient solution; conducting treatment with the reducing calcium ingredient solution; preserving the valve samples; conducting in-vitro calcification reaction with the calcium-containing plasma solution; and detecting calcium content by atomic absorption spectrum.