Biomarker Separation Workflow for Unified Sediment Analysis
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Solution Overview
Problem
Conventional methods for separating and analyzing biomarkers from sediments, source rocks, or reservoir rocks require different separation procedures based on the type of biomarker, which is inefficient and cumbersome.
Innovation Solution
A method is developed to simultaneously separate and analyze various types of biomarkers through a single process, involving extraction, removal of inorganic matter and asphaltenes, classification into saturated hydrocarbons, aromatic hydrocarbons, and NSO compounds, and detection using GC or GC/MS.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional separation procedures are used for different biomarker types, then each biomarker can be isolated with appropriate precision, but the analysis process becomes cumbersome and inefficient
Solution Approach 1:
The patent applies universality by developing a single analytical system that can handle multiple biomarker types (saturated hydrocarbons, aromatic hydrocarbons, and NSO compounds) through one unified procedure. The system uses a combination of solvent extraction, silica gel chromatography, and GC-MS that universally processes all three biomarker categories without requiring separate specialized methods for each type.
Solution Approach 2:
The patent merges multiple separation and analysis steps into an integrated workflow. The combination of solvent extraction, silica gel column chromatography with gradient elution, and GC-MS detection creates a consolidated procedure that replaces multiple conventional separate methods, thereby improving efficiency while maintaining precision.
2Measurement precision
If different separation procedures are used for each biomarker type, then analysis accuracy is maintained, but the complexity of the overall process increases
Solution Approach 1:
The patent applies segmentation by dividing the complex mixture of biomarkers into distinct fractions using silica gel column chromatography with gradient elution. The process segments saturated hydrocarbons, aromatic hydrocarbons, and NSO compounds into separate collectible fractions, allowing each to be analyzed individually with high accuracy while simplifying the overall procedure compared to handling the complete mixture.
3Adaptability or versatility
If multiple separate analysis procedures are employed, then comprehensive biomarker coverage is achieved, but the time required for complete analysis increases
Solution Approach 1:
The patent implements continuity of useful action by establishing an uninterrupted analytical workflow from sample extraction through chromatographic separation to GC-MS detection. The gradient elution process continuously separates different biomarker types in sequence without requiring intermediate interruptions or reconfiguration, enabling comprehensive coverage of all biomarker types within a single continuous operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the efficient and unified separation and analysis of multiple biomarker types from geological samples, enhancing the accuracy and efficiency of environmental and geological studies.
Implementation Method 1
extracting organic matter from an original sample
Implementation Method 2
removing inorganic matter from a sample obtained in step (a)
Implementation Method 3
removing asphaltene from a sample obtained in step (b) by mixing a non-polar organic solvent with the sample
Implementation Method 4
detecting components included in each of the group containing saturated hydrocarbons, the group containing aromatic hydrocarbons, and the group containing NSO compounds
Implementation Method 5
GC/MS
Data Source
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AI summary
The present disclosure provides a method for separating and analyzing biomarkers from sediments, source rocks, or reservoir rocks. Conventional techniques require different separation and analysis procedures depending on the type of biomarker, leading to increased process complexity and cost. To address these limitations, the present disclosure enables the simultaneous separation and analysis of various types of biomarkers through a single process, thereby improving efficiency and reducing operational costs.