Biomolecule Measuring Device Noise Reduction via Temporal Signal Separation
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Solution Overview
Problem
Biomolecule measuring devices using semiconductor sensors face challenges such as increased measuring time due to the need for long ultraviolet irradiation to remove trapped charges, impractical calibration for arrayed ISFETs, and lengthy background processing that decreases measurement accuracy and increases analysis time.
Innovation Solution
A biomolecule measuring device that generates a trigger for the reagent to react with a sample after starting reagent transmission, allowing for the separation of incorporation signals from background noise through temporal separation and simple calculations, reducing computational loads and data requirements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If ultraviolet irradiation is used to remove trapped charges from ISFET devices, then measurement accuracy is improved, but measuring time is significantly increased
Solution Approach 1:
The patent applies preliminary action by performing UV irradiation to remove trapped charges before the actual measurement process begins. This pre-treatment step ensures that the ISFET devices start in an optimized state with minimal charge interference, thereby improving measurement accuracy without adding time during the critical measurement phase. The background processing including UV irradiation is completed in advance, so it does not extend the actual measurement time.
2Measurement precision
If background processing is performed for all measured data in parallel measurements, then measurement accuracy is improved, but analyzing time is significantly increased
Solution Approach 1:
The patent extracts the background signal processing from the main measurement data analysis flow. By separately measuring and processing background signals in dedicated background wells, and then subtracting these from the total signals, the patent simplifies the computational burden. This extraction approach maintains measurement accuracy while significantly reducing analyzing time, especially for large-scale parallel measurements involving millions of ISFETs.
3Measurement precision
If ISFET devices are used to detect ion concentration changes, then biomolecule detection capability is improved, but signals from reagent replacement overlap with target signals
Solution Approach 1:
The patent segments the measurement process into distinct components by using separate background wells that contain only reagent solutions without biomolecules. This segmentation allows the background signal from reagent replacement to be measured independently and then subtracted from the total measurement signal, effectively separating the target signal from the harmful background interference while maintaining the detection capability of ISFET devices.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively reduces measurement noise and improves measurement accuracy by simplifying the process of removing drift, offset, and background signals, thereby shortening analysis time and enhancing data handling efficiency.
Implementation Method 1
ISFET is a device that measures interface potentials induced on ion sensitive layers
Implementation Method 2
a method to irradiate ultraviolet ray to provide electric charge with energy, thereby withdrawing the charge from the device
Data Source
AI summary
Provided is a biomolecule measuring device capable of effectively reducing measurement noise occurring when measuring a biomolecule sample using a semiconductor sensor. This biomolecule measuring device generates a trigger to react a sample with a reagent after starting to send the reagent onto the semiconductor sensor that detects ion concentration (see FIG. 7).