Bioparticle Analysis Capturing Secreted Substances
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Solution Overview
Problem
Current methods for analyzing bioparticles in populations, such as cell populations, fail to effectively capture and analyze secreted substances and intracellular molecules simultaneously, especially in diverse cell populations like immune cell populations, leading to incomplete understanding of cell interactions and functions.
Innovation Solution
A bioparticle analysis method involving a preparation step with a first capturing substance that binds secreted substances, followed by a second capturing step with a second capturing substance, allowing for the identification and analysis of secreted substances while maintaining the first capturing substance bound to the bioparticle, enabling single cell resolution and comprehensive analysis of interparticle interactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods are used to analyze bioparticle populations, then the analysis can be performed, but the ability to simultaneously capture and analyze secreted substances and intracellular molecules is insufficient
Solution Approach 1:
The patent divides the analysis into separate capturing steps: a first capturing step for secreted substances using a first capturing substance, and a second capturing step for intracellular molecules using a second capturing substance. This segmentation allows simultaneous capture of different molecular types while maintaining single-cell resolution, resolving the contradiction between measurement precision and information completeness.
Solution Approach 2:
The patent employs a nested structure where the first capturing substance and second capturing substance are both associated with the same bioparticle population, allowing nested capture of multiple molecular compartments (extracellular and intracellular) within the same analytical framework, thereby improving information completeness without sacrificing precision.
2Measurement precision
If single cell analysis is performed, then detailed cell-type specific information can be obtained, but the complexity of the analysis process increases
Solution Approach 1:
The patent performs preliminary capturing of secreted substances and intracellular molecules before final analysis. By pre-capting the molecules in their respective compartments using the first and second capturing substances, the complex analysis process is broken down into manageable steps, reducing the overall complexity while maintaining single-cell resolution.
3Loss of information
If comprehensive analysis of cell interactions is performed, then understanding of cell functions improves, but the difficulty of detecting and measuring increases
Solution Approach 1:
The patent introduces the first capturing substance and second capturing substance as intermediary molecules that facilitate the detection of secreted substances and intracellular molecules, respectively. These intermediaries simplify the detection process by providing specific binding sites, thereby reducing the difficulty of detecting and measuring cell interaction components while improving understanding of cell functions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method enables detailed analysis of cell types, secreted substances, and intracellular molecules within cell populations, providing insights into cell interactions and functions, thereby improving the understanding and optimization of cell therapeutic agents.
Implementation Method 1
a first capturing step of binding a secreted substance generated by placing the bioparticle population under a predetermined condition, to the first capturing substance
Implementation Method 2
a second capturing step of binding the secreted substance bound to the first capturing substance, to a second capturing substance configured to capture the secreted substance
Data Source
AI summary
An object of the present invention is to provide a technique for analyzing a bioparticle that is included in a bioparticle population and that is in a state in which an influence of the interparticle interaction is reflected.The present disclosure provides a bioparticle analysis method including: a preparation step of preparing a bioparticle population including a bioparticle to which a first capturing substance configured to capture a secreted substance is bound; a first capturing step of binding a secreted substance generated by placing the bioparticle population under a predetermined condition, to the first capturing substance; and a second capturing step of binding the secreted substance bound to the first capturing substance, to a second capturing substance configured to capture the secreted substance. The present disclosure also provides a reagent kit for bioparticle analysis used in the analysis method, and a bioparticle analysis system used for executing the analysis method.