Bioparticle Capture Structure for Image–Molecule Association
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Solution Overview
Problem
Existing methods fail to accurately associate morphological information with molecular information in single cell analysis, leading to high costs and difficulties in connecting captured image information with molecular information due to random encapsulation of barcode beads.
Innovation Solution
A bioparticle analysis system with a capturing device that immobilizes a cleavable linker, molecule capturing sequence part, and barcode sequence part, along with an information processing device to associate morphological and molecular information using barcode sequences.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If barcode beads are randomly encapsulated in microwells for single cell analysis, then the number of cells that can be analyzed increases and analysis cost decreases, but it becomes difficult to connect captured image information with molecular information for each well
Solution Approach 1:
The patent introduces a positioning structure (such as a patterned substrate or fiducial markers) as an intermediary element that mediates between the randomly encapsulated barcode beads and the imaging system. This positioning structure provides reference points that enable the imaging system to locate and associate molecular information from specific wells with corresponding morphological image data, solving the information connection problem while maintaining high-throughput random encapsulation
2Measurement precision
If a 96 well or 384 well plate is used for single cell analysis, then morphological information and molecular information can be integrated at a single cell level, but the number of cells that can be analyzed is limited to about several hundred and sequencing cost for each bioparticle is high
Solution Approach 1:
The patent divides the analysis system into two independent but correlated components: (1) a high-density microwell plate that enables random encapsulation of many cells with barcode beads for cost-effective molecular analysis, and (2) an imaging system that captures morphological information. By segmenting the functions and using the positioning structure to correlate results, the system achieves both high cell throughput and accurate single-cell level data integration
Solution Approach 2:
The positioning structure serves multiple functions: it acts as a reference for the imaging system to locate wells, provides spatial coordinates for associating image data with molecular data, and enables the system to handle both high-density microwell formats and traditional plate formats. This multi-functionality allows the system to achieve high productivity while maintaining measurement precision
Data Source
AI summary
Provided is a technique capable of associating morphological information with molecular information with high accuracy.The present technology provides a bioparticle analysis system, and the like, including: a capturing device that captures a bioparticle via a bioparticle capturing part on a surface on which a cleavable linker, the bioparticle capturing part, a molecule capturing sequence part, and a barcode sequence part are immobilized via the linker; and an information processing device that associates morphological information regarding the bioparticle obtained on the basis of captured image information with information regarding a molecule obtained on the basis of a barcode sequence part assigned to the molecule derived from the bioparticle captured by the molecule capturing sequence part.