3D Bioprinted Cell-Based Fish Muscle With W-Shaped Texture
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Solution Overview
Problem
Existing cell-based fish production techniques struggle to mimic the complex microstructure and texture of actual fish muscle, leading to uneven muscle tissue and limited mechanical properties.
Innovation Solution
A method using 3D bioprinting technology to mix fish cells with an edible bioink, print a laminated structure, and culture it with a crosslinking agent to create a cell-based fish with fish muscle shape-mimicking muscle, adjusting cell environment and applying shear stress for alignment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional scaffold production technology (salt leaching and gas forming) is used to carry cells, then cell-based fish can be produced, but the muscle tissue becomes uneven and the complex microstructure of actual fish muscle cannot be mimicked
Solution Approach 1:
The patent changes the production method from conventional scaffold-based approaches to 3D bioprinting technology, transforming the physical and chemical parameters of the process to achieve precise control over cell placement and tissue formation, thereby producing uniform muscle tissue with complex microstructure
Solution Approach 2:
The patent transitions from two-dimensional scaffold surfaces to three-dimensional bioprinted structures, enabling the creation of complex microstructures that mimic actual fish muscle architecture while maintaining uniform tissue distribution through precise spatial control
2Quantity of substance
If scaffolds with large porosity are used to support cells, then cell attachment space is provided, but mechanical properties deteriorate and muscle content is limited
Solution Approach 1:
The patent employs composite bioinks combining multiple materials with complementary properties, creating a structure that provides sufficient cell attachment space while maintaining enhanced mechanical strength that scaffold-based systems cannot achieve
Solution Approach 2:
The patent applies different material properties and structural characteristics to different regions of the bioprinted construct, optimizing local areas for cell attachment while other areas provide structural support, thereby achieving both high cell content and strong mechanical properties
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces a cell-based fish that aligns muscle cells to mimic the 'W' shape of real fish muscle, achieving actual fish muscle shape and texture, with improved cell differentiation and organization.
Implementation Method 1
printing the cell printing ink in a fish muscle-mimic pattern to form a laminated structure
Implementation Method 2
adding a crosslinking agent to the laminated structure
Implementation Method 3
adjusting cell environment and applying shear stress for alignment
Data Source
AI summary
Provided are a method for producing an edible cell-based fish that can realize actual fish muscle shape and texture, and a cell-based fish prepared thereby. The method for producing cell-based fish containing fish muscle shape-mimicking muscle using 3D bioprinting technology includes the steps of: mixing cells for cultured fish and an edible bioink; printing the cell printing ink in a fish muscle-mimic pattern to form a laminated structure; adding a crosslinking agent to the laminated structure; and culturing the laminated structure to which the crosslinking agent is added into a fish muscle tissue.