Bioprocess Sampling Container With Sealable Removable Portions
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Bioprocessing systems face challenges in automated, aseptic, and traceable sample removal without product variability, contamination, or damage, particularly in systems like the WAVE Bioreactor, where manual processes are user-intensive and prone to contamination and variability.
Innovation Solution
A consumable container with sealable, removable portions allows for automated, aseptic sampling without stopping product movement, using heat-sealable materials like EVA, PVC, or PE, and integrating analytical capabilities directly into the sampling process.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If manual sampling is performed in a WAVE Bioreactor, then the sample can be obtained, but the product movement must be stopped and contamination risk increases
Solution Approach 1:
The bioreactor system is segmented into a main reaction chamber and a separate sampling loop. The sampling loop can be isolated and operated independently, allowing sample collection without interrupting the main product movement and mixing in the bioreactor chamber.
Solution Approach 2:
A sampling loop acts as an intermediary between the bioreactor and the sampling system. This intermediate component receives product from the bioreactor, allows controlled sampling operations, and returns product to the bioreactor, thereby mediating the sampling process without requiring cessation of main product flow.
2Quantity of substance
If manual sampling with syringe is used, then sample can be extracted, but contamination and product damage occur due to uncontrolled interface
Solution Approach 1:
The sampling loop serves as a controlled intermediary environment where sampling occurs. It provides a sealed interface between the bioreactor and external sampling equipment, eliminating uncontrolled exposure and reducing contamination and damage risks.
Solution Approach 2:
The manual syringe aspiration mechanism is replaced with a controlled pumping system that operates within the sealed sampling loop. This substitution provides precise control over sample extraction, eliminating the uncontrolled mechanical interface problems associated with manual syringe use.
3Extent of automation
If automated sampling system is implemented, then user variability is reduced, but system complexity increases
Solution Approach 1:
The automated sampling system is segmented into modular components: a sampling loop, pumping system, and analysis interface. This segmentation allows automation to be implemented in a structured way, reducing overall system complexity compared to a fully integrated automated system.
Solution Approach 2:
The sampling loop serves multiple functions: it acts as a transfer medium between bioreactor and analysis system, provides a controlled sampling environment, enables automated operation, and facilitates product return. This multi-functionality reduces the need for separate dedicated components, thereby reducing system complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Ensures reliable, automated sampling that prevents contamination and damage, allows precise volume control, and integrates analysis directly into the sampling process, reducing user variability and costs.
Implementation Method 1
using heat-sealable materials like EVA, PVC, or PE
Data Source
AI summary
A consumable container for filling with a fluid in a bioprocessing process. The container comprises one or more sealable, removable portions, such that one or more samples of the fluid may be taken by sealing and removing one or more of said portions.