Bioreaction Container Pneumatic pH Control and Segmented Sampling
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Solution Overview
Problem
Conventional bioreactor devices face challenges in compactness, ease of assembly, sample collection, and pH control, which limits their effectiveness for small-scale laboratory tests and optimal culture conditions.
Innovation Solution
A bioreaction container design featuring a culture chamber with a filter cap for sample collection, a gas introduction system, and an acidity/basicity adjustment mechanism using pneumatic pressure, allowing for compact assembly and customizable pH control.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If the reaction tank is completely sealed with firmly coupled cover, then contamination is prevented, but sample collection becomes difficult and requires separation of cover which may cause contamination
Solution Approach 1:
The sealing structure is divided into a main sealing portion and a separate sampling portion. The sampling portion includes a sampling cap that can be opened independently from the main cover, allowing sample collection without compromising the overall seal integrity or requiring separation of the entire cover assembly.
Solution Approach 2:
A sampling valve or sampling port acts as an intermediary component between the sealed reaction tank interior and the external sampling environment. This intermediary allows selective access for sample withdrawal while maintaining the seal between the culture medium and the external environment.
2Adaptability or versatility
If conventional bioreactor devices are used, then culture functions are provided, but device size is large and assembly is complex
Solution Approach 1:
Multiple functional components are merged into an integrated assembly: the chamber cover portion integrates the sealing function, the gas introduction portion combines gas supply and mixing capabilities, and the acidity/basicity adjustment portion merges pH control with the cover structure. This integration reduces the number of separate parts and simplifies assembly while maintaining all necessary culture functions.
Solution Approach 2:
The chamber cover portion serves multiple functions simultaneously: it provides sealing, houses gas introduction components, enables pH adjustment, and facilitates sample collection. This multi-functionality reduces the overall device complexity by eliminating the need for separate components for each function.
3Productivity
If pH adjustment is not integrated, then device structure is simpler, but pH control requires external equipment and is less efficient
Solution Approach 1:
The acidity/basicity adjustment portion is merged with the chamber cover portion, integrating pH control functionality directly into the cover structure. This integration allows pH adjustment to be performed in-situ without requiring external equipment, improving control efficiency while adding only one integrated component assembly.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables compact, versatile, and efficient bioreaction containers for small-scale laboratory tests, facilitating rapid and optimal culture conditions while preventing contamination and external substance ingress.
Implementation Method 1
an acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution
Data Source
AI summary
A bioreaction container including a culture chamber configured to contain a culture solution and a life form in an inner space, the culture chamber having an open upper end; a chamber cover portion coupled to the upper end of the culture chamber, the chamber cover portion having a protruding tube provided on one side thereof so as to communicate with the inner space; a filter cap coupled to the protruding tube in an attachable/detachable manner so as to open/close the protruding tube; a gas introduction portion configured to penetrate the chamber cover portion and to communicate with the inner space so as to supply a predetermined gas into the inner space; and an acidity/basicity adjustment portion installed on the chamber cover portion while containing an adjustment solution that adjusts pH of the culture solution such that the adjustment solution is discharged into the inner space by pneumatic pressure.