Bioreactor Analysis Chips for High-Throughput Antigen Affinity Screening
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Solution Overview
Problem
The existing methods for analyzing antibody-specific DNA libraries are costly and time-consuming due to the need for manual colony picking and additional steps to verify physiological reactions, resulting in low throughput and high costs.
Innovation Solution
A method involving bioreactors formed on a substrate with structures that interact to hold biological samples, allowing for high-throughput analysis and extraction of samples with antigen-specific affinity through patterning technology, eliminating the need for additional verification steps.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If manual colony picking and additional verification steps are used, then measurement precision of physiological reaction is improved, but productivity and throughput deteriorate
Solution Approach 1:
The patent combines the culturing function and physiological reaction verification function into a single integrated bioreactor structure. The bioreactor simultaneously supports cell growth and enables real-time monitoring of physiological reactions through integrated measurement systems, eliminating the need for separate verification steps and thereby increasing throughput while maintaining measurement precision.
Solution Approach 2:
The bioreactor is designed as a multi-functional platform that performs multiple operations including cell culturing, physiological reaction monitoring, and data analysis within a single device. This universal design allows the system to handle both low-complexity and high-complexity analyses without requiring additional equipment or steps, thus improving productivity.
2Manufacturing precision
If manual colony picking is used, then manufacturing precision of sample isolation is improved, but loss of time increases
Solution Approach 1:
The patent replaces manual mechanical colony picking with an automated system that uses the bioreactor's integrated measurement and control mechanisms. The system automatically identifies and isolates colonies based on physiological reaction data, eliminating manual intervention and significantly reducing the time required for sample isolation while maintaining precision through automated control.
Solution Approach 2:
The bioreactor performs preliminary culturing and physiological reaction assessment within the same device before isolation is needed. By pre-characterizing colonies through integrated monitoring, the system prepares sample identification data in advance, allowing for rapid and precise isolation without time-consuming manual examination steps.
3Reliability
If additional verification steps are performed, then reliability of antigen-specific affinity analysis is improved, but device complexity increases
Solution Approach 1:
The patent merges the verification function into the bioreactor's integrated measurement system. The bioreactor simultaneously performs culturing and physiological reaction monitoring using integrated sensors and measurement tools, eliminating the need for separate verification equipment and steps. This reduces device complexity while maintaining reliability through continuous monitoring.
Solution Approach 2:
The bioreactor is designed as a universal platform that handles both simple culturing and complex physiological reaction analysis within the same device. By integrating multiple functions including culturing, monitoring, and analysis, the system achieves reliable antigen-specific affinity analysis without requiring additional specialized equipment or procedural steps.
Data Source
AI summary
Provided are a method of analyzing biological samples, an analytical chip and an analytical system comprising same, the method comprising the steps of: introducing structures for assisting the formation of bioreactors on a substrate; providing a plurality of bioreactors on the substrate by means of forming the bioreactors, which are holding biological samples, on the peripheral parts of the respective structures so as to come in contact with the structures in at least one region; and checking a change in the biological samples present in the bioreactors.