Bioreactor Sliding Support Assembly for Cell Detachment
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Solution Overview
Problem
Existing methods for separating adherent cells from culture supports using chemical substances like trypsin risk damaging cells and result in low recovery rates due to the interference with cell adhesion proteins and the need for prolonged exposure.
Innovation Solution
A bioreactor design that applies a physical impact to detach cells from supports using a sliding support assembly guided by long hole guide grooves and protruding elements, allowing for both indirect and direct physical shocks to facilitate cell separation without chemical damage.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of operation
If chemical substances such as trypsin are used to separate cells from the support, then cells can be detached from the support, but cell proteins may be destroyed due to prolonged exposure
Solution Approach 1:
The patent replaces the chemical separation method (trypsin) with a mechanical separation method. The support assembly is designed to slide between guide grooves, generating physical impact that detaches cells from the support through mechanical force rather than chemical treatment, thereby avoiding cell protein destruction while achieving effective cell separation
Solution Approach 2:
The patent changes the separation mechanism from chemical parameter (trypsin concentration and exposure time) to mechanical parameter (sliding distance and impact force). By adjusting the sliding distance of the support assembly within the guide grooves, the mechanical impact parameter is controlled to achieve cell detachment without chemical damage
2Object-affected harmful factors
If exposure time to chemical substances such as trypsin is shortened to prevent cell protein destruction, then cell damage is reduced, but the recovery rate of cells decreases
Solution Approach 1:
The patent substitutes chemical separation with mechanical separation through the sliding support assembly. The physical impact generated by sliding the support between guide grooves achieves complete cell detachment without chemical exposure, thereby maintaining high cell recovery rate while preventing cell protein destruction
Solution Approach 2:
The patent employs periodic sliding motion of the support assembly within the guide grooves. This periodic mechanical action repeatedly applies impact force to detach cells efficiently, achieving high recovery rates without the need for prolonged or repeated chemical treatment
3Productivity
If a sliding support assembly guided by guide grooves is used to apply physical impact, then cell separation efficiency is improved, but device complexity increases
Solution Approach 1:
The patent segments the bioreactor into distinct functional components: a fixed housing with guide grooves and a movable support assembly. This segmentation allows the support assembly to slide independently between the guide grooves, generating controlled physical impact for cell separation while keeping the overall device structure organized and manageable
Solution Approach 2:
The guide grooves serve as an intermediary element between the fixed housing and the movable support assembly. These grooves guide the sliding motion and transmit mechanical force, enabling cell separation through physical impact while simplifying the connection between components and reducing overall device complexity
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach increases the recovery rate of adherent cells by minimizing protein damage and enhancing the efficiency of cell detachment, enabling higher yields through physical separation methods.
Implementation Method 1
a pair of guide grooves formed by recessing an inner surface of the housing to guide the sliding movement of the support assembly when the support assembly is moved by an external force so that a physical impact is applied to the support assembly
Data Source
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AI summary
Provided is a bioreactor for adherent cells. The bioreactor for adherent cells according to one embodiment of the present invention comprises: a housing including a housing body, which has an enclosed shape and has an inner space open on one side, and a cover detachably coupled to the housing body so as to cover the open side of the inner space; a support assembly including a plurality of supports, which are formed as plate-shaped members having a predetermined area so that cells to be cultured can be attached to at least one surface, and which are disposed at intervals in multiple stages along one direction, and a plurality of fastening bars fastening the plurality of supports; and a pair of guide grooves which are formed recessed in the inner surface of the housing to guide the sliding of the support assembly so that a physical impact can be applied to the support assembly when the support assembly is moved by an external force.