Bioreactor Vibration Layout for Uniform Cell Separation
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Solution Overview
Problem
Existing bioreactors face inefficiencies in cell separation due to uneven transmission of ultrasonic vibrations, leading to increased costs and time consumption for desired cell separation.
Innovation Solution
A bioreactor design that includes a stirring shaft with an inner vibrating member and an outer vibrating member, both connected to an ultrasonic generator, to evenly transmit vibrations throughout the cell culture solution, combined with a built-in or external cell culture module to enhance cell separation efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If ultrasonic vibration is applied through a single container wall, then cell separation is achieved in the contact region, but vibration transmission is uneven and incomplete across the entire solution volume
Solution Approach 1:
The single ultrasonic source is divided into multiple ultrasonic generators (first and second ultrasonic generators) positioned at different locations on the container. This segmentation allows vibration to be applied at multiple points simultaneously, ensuring uniform vibration transmission throughout the entire solution volume and achieving complete cell separation without dead zones.
Solution Approach 2:
Multiple ultrasonic generators are combined to work simultaneously on the same solution volume. The first ultrasonic generator applies vibration through one container wall while the second ultrasonic generator applies vibration through another wall, merging their effects to achieve uniform and complete vibration distribution for enhanced cell separation efficiency.
2Productivity
If excess target substance is processed to ensure complete cell separation, then desired cell separation is achieved, but costs and time consumption increase
Solution Approach 1:
By segmenting the vibration source into multiple ultrasonic generators positioned strategically on the container, the system achieves complete cell separation throughout the entire solution volume in a single processing cycle. This eliminates the need to process excess material to compensate for incomplete separation in single-source configurations.
Solution Approach 2:
Multiple ultrasonic generators operate simultaneously and continuously throughout the processing period, ensuring that every region of the solution receives adequate vibration exposure. This continuous and uniform action achieves complete cell separation without requiring extended processing times or additional material cycles.
3Productivity
If multiple ultrasonic generators are used to improve vibration distribution, then cell separation efficiency increases, but device complexity increases
Solution Approach 1:
The system is segmented into multiple independent ultrasonic generator units that can be positioned at standard locations on the container. Each generator is a separate, modular component that can be independently controlled and maintained, making the increased complexity manageable through standardization and modularity.
Solution Approach 2:
Each ultrasonic generator serves multiple functions: it provides vibration for cell separation, can be positioned at standard container locations, and operates independently to cover different solution volumes. This multi-functionality justifies the added components by maximizing their utility and reducing overall system complexity through standardized designs.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Achieves smooth and efficient cell separation across the entire solution volume, reducing costs and time by ensuring uniform vibration distribution.
Implementation Method 1
the ultrasonic generator is driven to vibrate the solution or target substance by ultrasonic waves to induce cell separation
Implementation Method 2
a stirring blade mounted on the stirring shaft, which rotates together with the stirring shaft and agitates the cell culture solution
Data Source
AI summary
The present invention relates to a bioreactor for cell culture. According to an embodiment of the present invention, vibration is provided to a cell culture solution (solution) to facilitate cell separation, and, since the vibration is transmitted to the entire cell culture solution without dead zones, cell separation efficiency is excellent.