Bioreactor Vessel Rigid Ledge for Automated Fluid Connection

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Solution Overview

Problem

Bioreactor systems face challenges in optimizing conditions for cell culture production due to the complexity of controlling multiple parameters, high operational costs, and the lack of suitable small-scale models that accurately replicate large-scale conditions, leading to inefficiencies and increased costs in product yield optimization and cell line screening.

Innovation Solution

The introduction of a bioreactor vessel design featuring a sideways-projecting rigid ledge with an externally-facing connection port and a conduit integrated into the vessel, allowing for direct fluid pathway establishment with a base station, eliminating the need for intermediary conduits and reducing contamination risks, and a system for simultaneous connection of multiple vessels to streamline experiment setup and reduce labor.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Ease of operation

If traditional bioreactor vessels are used with separate connection components, then fluid pathways can be established, but the setup process becomes complex and time-consuming with increased contamination risks

Engineering Contradiction:
Improvefluid pathway setupVSAvoidconnection components
Core Design Contradiction:
Ease of operationVSDevice complexity

Solution Approach 1:

The patent merges the connection port and conduit into a single integrated component that is pre-attached to the bioreactor vessel. The rigid ledge with the connection port provides a stable mounting surface, and the conduit is directly connected to it, eliminating the need for separate connection components and manual assembly steps.

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The conduit is pre-positioned and pre-connected to the connection port on the rigid ledge before the vessel is placed in the bioreactor system. This preliminary assembly ensures proper alignment and connection readiness, reducing setup time and contamination risks during operation.

Inventive Principle:
Principle #10Preliminary action

2Productivity

If multiple bioreactor vessels are processed individually, then each can be optimized, but the overall throughput and productivity are limited

Engineering Contradiction:
Improveexperiment throughputVSAvoidsetup time per vessel
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The standardized rigid ledge with connection port design creates a universal interface that can accommodate multiple vessels simultaneously in the bioreactor system. This multi-functional design allows parallel processing of multiple vessels with the same connection mechanism, significantly increasing throughput.

Inventive Principle:
Principle #6Universality (Multi-functionality)

3Adaptability or versatility

If flexible conduits are used for fluid connections, then adaptability is improved, but connection stability and alignment precision deteriorate

Engineering Contradiction:
Improveconnection flexibilityVSAvoidconnection alignment
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The rigid ledge acts as an intermediary structure between the vessel and the external fluid supply system. It provides a stable, precisely positioned connection port that ensures accurate alignment, while still allowing for some flexibility in the overall system configuration.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentEP2880148B1Bioreactor vessels and associated bioreactor systems
Publication Date: 2021.07.21 THE AUTOMATION PARTNERSHIP (CAMBRIDGE) LTD
  • EP2880148B1 patent drawingFigure 1~5
  • EP2880148B1 patent drawingFigure 2~3A
  • EP2880148B1 patent drawingFigure 4~14

AI summary

A bioreactor vessel (100) is provided with a rigid ledge projecting to a side thereof and defining fluid conduits (136a-c) between three respective externally-facing ports (132a-c) and a vessel chamber (105). A bioreactor system includes a cell culture module (10) having a receiving station (14) in which the vessel (100) is received, in use. Three fluid connection ports (314a-c) are located adjacent to the receiving station (14) and are in fluid connection with associated gas and/or liquid input lines (302a-c, 316, 318) via a valve assembly (300). When the vessel (100) is inserted into the receiving station, the rigid nature of the ledge and the defined positions of the receiving station connection ports (314a-c) mean that the externally-facing ports (132a-c) on the vessel are brought into registration with the receiving station connection ports (314a-c), thereby forming respective fluid connections automatically, without having to manually couple fluid lines on the vessel (100) to ports on the cell culture base (12).