Biosensing Membrane Electron Transfer via Redox Mediators
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Solution Overview
Problem
Existing biosensors face challenges in achieving stable and efficient electron transfer between redox active sites of oxidoreductases and electrodes, limiting their detection efficiency and stability, especially when implanted in living bodies.
Innovation Solution
Electrochemically activating and modifying oxidoreductases using ruthenium or osmium complexes, followed by cross-linking with glutaraldehyde or polyethylene glycol diglycidyl ether, and coating them on electrodes to form a durable biosensing membrane that enhances electron transfer and stability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a redox active molecule is introduced into the biosensing membrane to establish an electron transfer channel, then electron transfer efficiency is improved, but the stability and durability of the biosensing membrane deteriorates
Solution Approach 1:
The patent introduces a redox active molecule as an intermediary substance between the oxidoreductase and the electrode. This mediator facilitates electron transfer from the redox active sites of the oxidoreductase to the electrode surface, solving the electron transfer efficiency problem while maintaining membrane stability through proper molecular selection and cross-linking
Solution Approach 2:
The patent creates a composite biosensing membrane structure comprising oxidoreductase, redox active molecules, and cross-linking agents. This composite material approach integrates multiple functional components that work synergistically to achieve both high electron transfer efficiency and long-term stability, with the cross-linking network providing structural integrity
2Reliability
If the oxidoreductase is modified with ruthenium or osmium complexes to enhance electron transfer, then electron exchange efficiency is improved, but the complexity of the preparation process increases
Solution Approach 1:
The patent modifies the oxidoreductase by introducing ruthenium or osmium complexes with specific coordination structures and redox potentials. These parameter changes in the molecular structure enable direct electron exchange with the electrode, improving efficiency while the systematic modification protocol manages process complexity
3Stability of the object's composition
If a chemical cross-linking agent is used to fix the membrane on the electrode, then membrane stability is improved, but the loss of biological activity of oxidoreductase may increase
Solution Approach 1:
The patent employs cross-linking agents such as glutaraldehyde or polyethylene glycol diglycidyl ether to create stable membrane structures. While cross-linking may partially reduce biological activity, the resulting membrane stability enables repeated detections and long-term implantation, providing overall system reliability
Solution Approach 2:
The patent applies cross-linking treatment to achieve sufficient membrane stability without complete cross-linking of all protein structures. This partial cross-linking approach maintains adequate biological activity while providing the mechanical and chemical stability needed for implantable devices
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method results in a stable and durable biosensing membrane with significantly improved electron transfer efficiency, suitable for repeated detections and long-term monitoring of target substances in living bodies, with catalytic oxidation efficiency enhanced by 140 times for glucose oxidase.
Implementation Method 1
electrochemically activating and modifying an oxidoreductase
Implementation Method 2
the electron exchange between the oxidoreductase and the electrode is an important step performed by a biosensor
Implementation Method 3
performing a cross-linking treatment using a chemical cross-linking agent
Data Source
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AI summary
A method for preparing a biosensing membrane: electrochemically activating and modifying an oxidoreductase, then performing a cross-linking treatment using a chemical cross-linking agent, and then coating on a surface of an electrode, thereby forming a biosensing membrane, wherein the chemical cross-linking agent is glutaraldehyde or polyethylene glycol diglycidyl ether. Also disclosed are a prepared biosensing membrane and monitoring device. The provided preparation method, or the biosensing membrane and monitoring device prepared by the preparation method are stable and durable, and may carry out a plurality of detections, and the foregoing biosensing membrane is particularly suitable to act as a biosensing membrane of a living body monitoring device.