Biosensor Detection Region With Separated Carrier Surface
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Solution Overview
Problem
Existing biosensors face challenges in efficiently detecting analytes due to premature reaction or mixing of reagents with the sensor surface, leading to inaccurate results and reduced analysis time.
Innovation Solution
A device with a detection region delimited by a carrier surface and a sensor surface, where the reagent is physically separated from the sensor surface, allowing for controlled mixing and reaction time, and featuring a capillary inlet for fluid transport and magnetic actuation for probe direction, enabling rapid and reproducible analyte detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Loss of time
If the reagent is deposited directly on the sensor surface, then the analysis time is reduced, but premature reaction or mixing of the reagent with the sensor surface occurs, disturbing the detection
Solution Approach 1:
The device is divided into separate functional zones: a reagent application zone (carrier surface) and a detection zone (sensor surface), physically separated by a distance. This segmentation allows the reagent to be applied without immediate contact with the sensor surface, preventing premature reactions while maintaining efficient detection timing.
Solution Approach 2:
The patent introduces an intermediate space (the detection region between carrier surface and sensor surface) that mediates the interaction between reagent and sensor. This intermediate zone allows controlled transport and mixing of reagent with sample fluid before reaching the sensor surface, ensuring proper reaction timing.
2Reliability
If the reagent is physically separated from the sensor surface, then premature reaction is prevented, but the device complexity increases
Solution Approach 1:
The carrier surface serves multiple functions: it holds the reagent, defines the detection region boundary, and provides a reference for fluid flow direction. The detection region itself serves as both a physical separator and a reaction chamber. This multi-functionality reduces the need for additional components, keeping the device relatively simple despite the separated architecture.
3Loss of time
If the detection region is minimized for rapid detection, then the analysis time is reduced, but the mixing of sample fluid with reagent is insufficient
Solution Approach 1:
The detection region is designed with specific local characteristics: it has sufficient volume to allow adequate mixing of sample fluid and reagent, yet maintains a compact overall size for rapid detection. The geometry and dimensions of the detection region are optimized to provide appropriate residence time and mixing conditions in this specific local zone.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution allows for fast, reliable, and reproducible analyte detection with improved mixing and reaction control, reducing the risk of premature reagent-sensor interactions and enabling miniaturization of the device while maintaining long shelf life.
Implementation Method 1
A sample fluid inlet is provided having an inlet opening in the detection region
Implementation Method 2
magnetic actuation for probe direction, enabling rapid and reproducible analyte detection
Data Source
AI summary
A system and method wherein components of a reagent such as labeled antibodies are separated from a biologically active sensor surface by depositing the reagent on a carrier surface distinct from a sensor surface in a detection region. The present device provides a short, well-defined and controlled, pre-incubation time between the particles of interest in the sample fluid and the reagent, thereby increasing the reproducibility by providing all components in one detection region such as a detection chamber.