Biosensor Hydrophilic Region Controls Reagent Alignment
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Solution Overview
Problem
Conventional biosensors experience variations in sensitivity due to changes in the contact area between the sample and the electrodes, leading to inconsistent measurement results, and face design restrictions with reagent coverage and electrode alignment.
Innovation Solution
A biosensor design featuring electrodes on an insulation substrate with a hydrophilic region having higher hydrophilicity than the surrounding area, where the reagent layer is specifically formed to ensure consistent contact with the electrodes, reducing positional deviations and production costs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the reagent layer is dissolved into the sample and an oxidation-reduction reaction proceeds, then the glucose concentration can be measured based on the current value, but the sensitivity varies due to variations in the contact area between the sample and the working electrode
Solution Approach 1:
The patent applies local quality by creating a hydrophilic region with different surface properties than the surrounding hydrophobic region. This localized hydrophilic area on the electrode surface ensures consistent wettability and contact area between the liquid sample and the working electrode, thereby stabilizing the sensitivity across different biosensors while maintaining the electrochemical measurement function.
2Reliability
If the working electrode is surrounded by the counter electrode and both are completely covered with the reagent, then the electrode configuration is complete, but design restrictions arise requiring insulation material to cover the reagent region overlapping with the lead section
Solution Approach 1:
The patent applies local quality by forming the reagent layer only on the hydrophilic region rather than covering the entire electrode surface. This localized reagent placement ensures that the reagent does not overlap with the lead section, eliminating the need for additional insulation material in that area while maintaining complete electrode configuration for reliable operation.
3Manufacturing precision
If masks are used during the production of the biosensor to control reagent placement, then the reagent layer can be precisely positioned, but the production cost increases
Solution Approach 1:
The patent applies self-service by using the hydrophilic region's inherent surface properties to automatically guide and confine the reagent layer formation. The hydrophilic region acts as a self-defining boundary that directs reagent placement without requiring external masks or complex alignment mechanisms, thereby achieving precise positioning while simplifying the manufacturing process and reducing costs.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The biosensor achieves stable contact areas between the reagent layer and electrodes, minimizing sensitivity variations among biosensors and reducing production costs by using a hydrophilic region to control reagent distribution and alignment.
Implementation Method 1
a hydrophilic region that is continuously formed on the electrodes, the hydrophilic region (i) having higher hydrophilicity than a surrounding region thereof
Implementation Method 2
a reagent layer comprising an enzyme and a mediator, the reagent layer being disposed on the hydrophilic region
Implementation Method 3
exchange of electrons is performed between substance to be measured and oxidoreductase, and the electrons then move via the electron transfer mediator to the electrode
Data Source
AI summary
The disclosure relates to a biosensor including electrodes, a hydrophilic region or layer, and a reagent layer that contains an enzyme and a mediator, and methods of producing thereof.