Biosensor Micropore Layer Nested in Foundation Throughhole
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Solution Overview
Problem
The high cost and low efficiency of fabricating biosensors for nanopore sequencing due to the need for precise devices like electron beam exposure and precision etching to create orifices of 10−9 meters in diameter, making mass production difficult.
Innovation Solution
A biosensor apparatus comprising a base substrate with a fluid channel layer, a foundation layer with a throughhole, and a micropore layer that extends through the foundation layer to connect to the fluid channel, allowing for the detection of target molecules by analyzing electrical signal changes as they pass through the micropore.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If electron beam exposure and precision etching are used to create orifices of 10^-9 meters in diameter, then measurement precision is improved, but device complexity and manufacturing cost increase
Solution Approach 1:
The patent divides the orifice formation process into multiple stages: first creating a larger through-hole in the foundation layer, then forming the micropore layer with the actual nanopore. This segmentation allows the complex nanopore fabrication to be broken down into simpler, more manufacturable steps that can be performed with less sophisticated equipment.
Solution Approach 2:
The micropore layer is nested within the foundation layer structure, where the micropore extends through the micropore layer and connects to the through-hole in the foundation layer. This nested configuration allows the nanopore to be formed within a pre-established structural framework, reducing the overall fabrication complexity.
2Measurement precision
If electron beam exposure and precision etching are used to create orifices of 10^-9 meters in diameter, then measurement precision is improved, but productivity decreases
Solution Approach 1:
By segmenting the orifice formation into foundation layer through-hole creation followed by micropore layer formation, the process becomes more amenable to batch fabrication and mass production techniques, improving productivity while maintaining the required detection precision.
Solution Approach 2:
The patent employs a disposable micropore layer that can be easily fabricated and replaced. This approach allows for high-volume production of biosensor apparatus without requiring expensive, complex fabrication equipment for each individual device, thereby improving productivity and enabling mass production.
3Manufacturing precision
If micropore layer extends into foundation layer throughhole and covers inner wall, then manufacturing precision is improved, but device complexity increases
Solution Approach 1:
The micropore layer is designed to extend into and cover only the inner wall of the foundation layer throughhole, providing localized precision where it is most needed for nanopore positioning, while maintaining simpler structures in other regions of the device.
Solution Approach 2:
The foundation layer through-hole is formed in advance before the micropore layer is deposited. This preliminary action provides a pre-established template that guides the micropore formation process, ensuring accurate positioning without requiring complex real-time control during micropore fabrication.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces the complexity and cost of fabricating biosensors, enabling mass production while maintaining the ability to detect target molecules with high precision by utilizing micropores of varying diameters to filter and analyze molecules such as DNA chains.
Implementation Method 1
the micropore layer extends into the foundation layer throughhole and at least partially covers an inner wall of the foundation layer throughhole
Implementation Method 2
allowing for the detection of target molecules by analyzing electrical signal changes as they pass through the micropore
Data Source
AI summary
A biosensor apparatus is provided. The biosensor apparatus includes a base substrate; a first fluid channel layer on the base substrate and having a first fluid channel passing therethrough; a foundation layer on a side of the first fluid channel layer away from the base substrate, a foundation layer throughhole extending through the foundation layer to connect to the first fluid channel; and a micropore layer on a side of the foundation layer away from the base substrate, a micropore extending through the micropore layer to connect to the first fluid channel through the foundation layer throughhole. The micropore layer extends into the foundation layer throughhole and at least partially covers an inner wall of the foundation layer throughhole.