Oxygen Tension Reduction in Electrochemical Biosensors
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Solution Overview
Problem
Diagnostic testing devices face interference from oxygen in bodily fluids, leading to erroneous results due to oxygen's interaction with electrochemical test strips, which is challenging to mitigate without affecting analyte analysis.
Innovation Solution
Incorporating an oxygen tension reducing agent, such as laccase or a platinum electrode, to convert oxygen to water, thereby reducing oxygen interference and using a mediator like ferrocyanide to facilitate accurate analyte detection, with a two-potential method to activate the platinum electrode for oxygen reduction and analyte detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If oxygen is present in the bodily fluid sample, then the electrochemical biosensor can operate with the mediator, but oxygen interference causes erroneous analyte detection results
Solution Approach 1:
The patent introduces an oxygen tension reducing agent that converts the harmful oxygen interference into a beneficial effect by reducing oxygen to water, thereby eliminating the harmful interference while maintaining the electrochemical detection function. This transforms the harmful oxygen presence into a resolved state where oxygen is converted to harmless water.
Solution Approach 2:
The patent employs an oxygen tension reducing agent as an intermediary substance between the oxygen and the electrochemical mediator. This intermediary component selectively reacts with oxygen to reduce its tension, protecting the main detection system from oxygen interference without affecting the analyte detection process.
2Measurement precision
If an oxygen tension reducing agent is added to eliminate oxygen interference, then analyte detection accuracy improves, but the device complexity increases
Solution Approach 1:
The patent combines the oxygen tension reducing agent with the electrochemical mediator in a single integrated test strip design. The reducing agent and mediator are merged into one device, allowing simultaneous oxygen reduction and analyte detection without requiring separate systems or multiple操作步骤.
Solution Approach 2:
The electrochemical biosensor is designed to perform multiple functions: it detects the analyte through the mediator while simultaneously reducing oxygen interference through the oxygen tension reducing agent. This multi-functional design eliminates the need for separate oxygen removal devices, maintaining simplicity while achieving both detection accuracy and interference elimination.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach effectively eliminates oxygen interference, ensuring accurate analyte detection in both venous and oxygenated blood samples by reducing oxygen tension, thereby improving the reliability of electrochemical biosensors.
Implementation Method 1
the oxygen tension reducing agent is laccase. In another alternative, the laccase reacts with the oxygen to produce water.
Implementation Method 2
the laccase reacts with the oxygen to produce water
Implementation Method 3
a mediator, the mediator configured to provide for the testing of the analyte by facilitating electron exchange between the analyte and the biosensor
Implementation Method 4
the oxygen tension reducing agent is a platinum electrode. Alternatively, the platinum electrode is activatable at an oxygen reducing voltage, which causes the platinum electrode to reduce oxygen interference
Data Source
AI summary
In one embodiment, a system for detecting an analyte in a bodily fluid sample includes an electrochemical biosensor, the electrochemical biosensor configured to receive the bodily fluid sample, test for the analyte, and produce an analyte level for the analyte. The system further includes a mediator, the mediator configured to provide for the testing of the analyte by facilitating electron exchange between the analyte and the biosensor. The system further includes an oxygen tension reducing agent, the oxygen tension reducing agent reducing interference of oxygen with the operation of the mediator.